Human IL-7 Antibody

(7 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-7 in Western blots. In ELISAs, does not cross-react with bovine FGF acidic, recombinant human (rh) G-CSF, rhGM-CSF, rhIL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, recombinant mouse IL-7, rhIL-8, porcine PDGF, rhTNF-alpha, rhTNF-beta, human TGF-beta 1, or porcine TGF-beta 1.
  • Source
    Monoclonal Mouse IgG1 Clone # 7417
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IL-7
    Accession # P13232
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑7 (Catalog # 207-IL)
    under non-reducing conditions only
    • Human IL-7 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IL‑7 Antibody (Catalog # MAB207)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IL‑7 Biotinylated Antibody (Catalog # BAF207)
  • ELISA Standard
     
    Recombinant Human IL-7 Protein (Catalog # 207-IL)
  • Neutralization
    Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). The Neutralization Dose (ND50) is typically 0.4-0.8 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑7.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL‑7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑7 Monoclonal Antibody (Catalog # MAB207). The ND50 is typically 0.4-0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-7

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4+ and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. ­IL-7 is expressed by adherent stromal cells from various tissues.

Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma  are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells, and bone marrow cells.

  • Long Name:
    Interleukin 7
  • Entrez Gene IDs:
    3574 (Human); 16196 (Mouse); 25647 (Rat)
  • Alternate Names:
    IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016;6(0):32320.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015;290(37):22338-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis.
    Authors: Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S
    J Immunol, 2013;190(10):5256-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
    Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
    Int. J. Cancer, 2009;125(5):1092-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  6. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  7. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Hamster, Human.
We have 2 reviews tested in 2 applications: ELISA, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
ELISA Human IL-7 Antibody MAB207
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Excellent
 ELISA Human Anonymous 11/17/2017
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ELISA Human IL-7 Antibody MAB207
ELISA: Human IL-7 Antibody [MAB207]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe antibody was used in an ELISA application. The R&D antibody AF-207 was used as the detection. IL-7 in human samples was measured.
  Very Good
 Flow Hamster Anonymous 11/04/2017
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Summary

ApplicationFlow Cytometry
Sample TestedCHO Chinese hamster ovary cell line
SpeciesHamster

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