Human IL-7 Antibody

Catalog # Availability Size / Price Qty
AF-207-NA
AF-207-SP
Human IL-7 Antibody in Data
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)

Human IL-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-7 in direct ELISAs and Western blots. In Western blots, detects recombinant mouse IL-7 but no cross-reactivity with recombinant rat IL-17.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-7
Asp26-His177
Accession # P13232
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND50) is typically 0.075-0.5 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Recombinant Human and Mouse IL-7 antibody by Western Blot. View Larger

Detection of Recombinant Human and Mouse IL‑7 by Western Blot. Western blot shows 25 ng of Recombinant Human IL-7 (Catalog # 207-IL), Recombinant Mouse IL-7 (Catalog # 407-ML) and Recombinant Rat IL-7 (Catalog # 7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. View Larger

Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL-7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND50is typically 0.075-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4+ and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. ­IL-7 is expressed by adherent stromal cells from various tissues.

Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma  are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Long Name
Interleukin 7
Entrez Gene IDs
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

Product Datasheets

Citations for Human IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion.
    Authors: Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo L, Marti L
    PLoS ONE, 2014;9(9):e106673.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
    Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW
    J. Immunol., 2005;175(11):7325-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005;174(4):2376-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Structure function analysis of interleukin 7: requirement for an aromatic ring at position 143 of helix D.
    Authors: vanderSpek JC, Sutherland JA, Gill BM, Gorgun G, Foss FM, Murphy JR
    Cytokine, 2002;17(5):227-33.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human IL-7 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human IL-7 Antibody
By Anonymous on 03/08/2018
Application: WB Sample Tested: Tissue Lysates Species: Human

Human IL-7 Antibody
By Anonymous on 11/17/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection paired with MAB207 as the capture in an ELISA. IL-7 in human samples were measured.


Human IL-7 Antibody
By Anonymous on 11/04/2017
Application: ChIP Sample Tested: 786-O human renal cell adenocarcinoma cell line Species: Human