|Detection of Recombinant Human and Mouse IL‑7 by Western Blot. Western blot shows 25 ng of Recombinant Human IL‑7 (Catalog # 207-IL), Recombinant Mouse IL‑7 (Catalog # 407-ML) and Recombinant Rat IL‑7 (Catalog # 7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.|
|Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL‑7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND50 is typically 0.02-0.04 µg/mL.|
IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4+ and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.
Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity.
IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.
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ELISA: Human IL-7 Antibody [AF-207-NA]
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