Human IL-7 R alpha /CD127 Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human
    (rh) IL‑10 R, rhIL‑2 R beta, rhIL‑5 R alpha, and rhIL‑6 R.
  • Source
    Monoclonal Mouse IgG1 Clone # 40131
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (Catalog # 306-IR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human
IL‑7 R alpha /CD127 Monoclonal Antibody (Catalog # MAB306) or
(B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). 
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-7 R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

  • References:
    1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
    2. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
    3. Goodwin, R.G. et al. (1990) Cell 60:941.
    4. Noguchi, M. et al. (1993) Science 262:1877.
    5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
    6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125.
    7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
    8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
    9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
    10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
    11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
    12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
    13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
    14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
    15. Jahn, T. et al. (2007) Blood 110:1840.
    16. Lai, L. et al. (2006) Blood 107:1776.
  • Long Name:
    Interleukin 7 Receptor alpha
  • Entrez Gene IDs:
    3575 (Human); 16197 (Mouse); 294797 (Rat)
  • Alternate Names:
    CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. CD56(bright) NK IL-7R? expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections
    Authors: CJ Judge, L Kostadinov, KE Sherman, AA Butt, Y Falck-Ytte, NT Funderburg, AL Landay, MM Lederman, SF Sieg, JK Sandberg, DD Anthony
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  2. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study
    PLoS Med, 2016;13(12):e1002200.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. The clinical correlation of regulatory T cells and cyclic adenosine monophosphate in enterovirus 71 infection.
    Authors: Wang, Shih-Min, Chen, I-Chun, Liao, Yu-Ting, Liu, Ching-Ch
    PLoS ONE, 2014;9(7):e102025.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
    Genes Immun, 2012;13(7):587-92.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  5. Functionally significant differences in expression of disease-associated IL-7 receptor alpha haplotypes in CD4 T cells and dendritic cells.
    Authors: Hoe E, McKay FC, Schibeci SD, Gandhi K, Heard RN, Stewart GJ, Booth DR
    J. Immunol., 2010;184(5):2512-7.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  6. Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
    Authors: Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J
    J. Biol. Chem., 2010;285(20):14898-908.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  7. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
    Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
    J. Immunol., 2009;182(12):7389-97.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  8. Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma.
    Authors: Srivastava BI, Srivastava MD
    Leuk. Res., 2005;29(7):771-83.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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