Human IL-9 Antibody Summary
Accession # P15248
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 Antibody. Recombinant Human IL‑9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF209). The ND50 is typically 2-5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLV-I) transformed T cell line that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, M07e. The cDNA encoding this cytokine was subsequently isolated by functional expression cloning and found to be similar to the mouse T cell growth factor III/P40. This human cytokine and its murine homologue are now designated as human and mouse IL-9. Besides HTLV-I or -II transformed T cell lines, rhIL-9 is also produced by activated human PBLs. Human IL-9 was also reported to be expressed by primary and cultured Hodgkin and Reed-Sternberg (H-RS) cells derived from Hodgkin’s disease patients, suggesting a possible role for rhIL-9 in the development of the pathophysiology of Hodgkin’s disease.
Human and murine IL-9 are also capable of enhancing in vitro survival of human T cell lines as well as synergizing with Epo to support erythroid colony formation in vitro. However, the mast cell enhancing activity associated with rmIL-9 has not yet been demonstrated in the human system and no human IL-9-dependent T cell clones have been identified.
The gene for rhIL-9 has been mapped to human chromosome 5. As in the mouse system, the human IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Human IL-9 contains four potential N-linked glycosylation sites and the native rhIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although murine IL-9 is active on human cells, human IL-9 is not active on mouse cells.
Citation for Human IL-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis.
Authors: Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P, Walls AF, Senechal S, Levitt RC, Darras J, Hamid Q, Wallaert B
J. Allergy Clin. Immunol., 2004;113(3):462-9.
Sample Types: Whole Tissue
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