Human IRF3 Antibody

Catalog # Availability Size / Price Qty
MAB4019
MAB4019-SP
Detection of Human IRF3 by Western Blot.
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Human IRF3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRF3 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 482205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IRF3
aa 206-427
Accession # Q14653
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IRF3 antibody by Western Blot. View Larger

Detection of Human IRF3 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IRF3 Monoclonal Antibody (Catalog # MAB4019) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of IRF3 antibody in Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IRF3 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IRF3 Monoclonal Antibody (Catalog # MAB4019, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF3

IRF3 (interferon response factor 3) is a 60 kDa member of the IRF family of proteins. Human IRF3 contains one DNA binding domain (aa 7‑107), a nuclear export signal (aa 139‑149) and multiple phosphorylation sites (aa 395‑407). Viral infection stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. Alternate splice forms may exist. One will show a deletion of aa 201‑327, a second will show the same deletion plus an alternate start site at Met147, and a third will show a 125 aa substitution for the C-terminal 100 aa (aa 328‑427). Over aa 206‑427, human IRF3 is 76% and 83% aa identical to mouse and pig IRF3, respectively.

Long Name
Interferon Regulatory Factor 3
Entrez Gene IDs
3661 (Human); 54131 (Mouse)
Alternate Names
interferon regulatory factor 3; IRF3; IRF-3

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