Human IRS1 Antibody

Catalog # Availability Size / Price Qty
MAB39782
MAB39782-SP
Detection of Human IRS1 by Western Blot.
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Human IRS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRS1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) IRS1 (aa 898-1066) and no cross-reactivity with rmIRS1 (aa 13-113), rmIRS3, or recombinant human IRS2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 614115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IRS1
Ala801-Ala1020 (predicted)
Accession # P35568
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IRS1 by Western Blot. View Larger

Detection of Human IRS1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IRS1 Monoclonal Antibody (Catalog # MAB39782) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRS1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators.  Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2.  Human IRS1 is 1242 amino acids (aa) in length.  It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225).  IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment.  Over aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse and pig IRS1, respectively.  

Long Name
Insulin Receptor Substrate 1
Entrez Gene IDs
3667 (Human); 16367 (Mouse); 25467 (Rat)
Alternate Names
HIRS-1; insulin receptor substrate 1; IRS1; IRS-1

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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