Detects human IRS1 in direct ELISAs.
In direct ELISAs, 100% cross-reactivity
with recombinant mouse (rm) IRS1 (aa 898-1066) and no cross-reactivity with
rmIRS1 (aa 13-113), rmIRS3, or recombinant human IRS2 is observed.
Monoclonal Mouse IgG1 Clone # 614115
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IRS1 Ala801-Ala1020 (predicted) Accession # P35568
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IRS1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IRS1 Monoclonal Antibody (Catalog # MAB39782) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRS1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Over aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse and pig IRS1, respectively.
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