Human IRS1 Antibody Summary
Accession # P35568
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IRS1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IRS1 Monoclonal Antibody (Catalog # MAB39782) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRS1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Over aa 801-1020, human IRS1 is 88%, 86% and 88% aa identical to dog, mouse and pig IRS1, respectively.
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