Human Kallikrein 3/PSA Antibody
R&D Systems | Catalog # MAB13442
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Capture (Matched Antibody Pair)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 181811
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Pro19-Pro261
Accession # P07288
Pro19-Pro261
Accession # P07288
Specificity
Detects human Kallikrein 3/PSA in sandwich immunoassays and in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Kallikrein 3/PSA Antibody
Detection of Human Kallikrein 3/PSA by Western Blot.
Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Kallikrein 3/PSA Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human prostate tissue
Sample: Human prostate tissue
Human Kallikrein 3/PSA Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Kallikrein 3/PSA
Additional Kallikrein 3/PSA Products
Product Documents for Human Kallikrein 3/PSA Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Kallikrein 3/PSA Antibody
For research use only
Citations for Human Kallikrein 3/PSA Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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