Human Kallikrein 3/PSA Antibody

Catalog # Availability Size / Price Qty
AF1344
AF1344-SP
Human Kallikrein 3/PSA Antibody in Western Blot
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Product Details
Citations (2)
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Human Kallikrein 3/PSA Antibody Summary

Species Reactivity
Human
Specificity
Detects human KLK3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) KLK2 is observed and less than 5% cross-reactivity with rhKLK1, rhKLK4, rhKLK5, rhKLK7, rhKLK8, rhKLK9, rhKLK10, rhKLK11, rhKLK12, rhKLK13, and rhKLK15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3
Ala18-Pro261
Accession # P07288
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5-1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human  Kallikrein 3/PSA by  Western Blot. View Larger

Detection of Human Kallikrein 3/PSA by Western Blot. Western blot shows lysates of Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE), DU145 human prostate carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 3/PSA at approximately 28-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Kallikrein 3/PSA by Western Blot. View Larger

Detection of Human Kallikrein 3/PSA by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Kallikrein 3/PSA at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Kallikrein 3/PSA  in LNCaP and MCF-7 Human Cell Lines. View Larger

Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines. Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF‑7 human breast cancer cell line (negative control, right panel) using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Kallikrein 3/PSA in Human Kidney Cancer Tissue. View Larger

Kallikrein 3/PSA in Human Kidney Cancer Tissue. Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 3/PSA

Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family (1). PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L) (2). A small portion of PSA “leaks” into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue (3). PSA has become a well established tumor marker that aids the diagnosis, staging, and follow up of prostate cancer.

The deduced amino acid sequence of human PSA consists of a signal peptide, a short pro region and a mature/active enzyme. The pro-enzyme is activated, possibly by active Kallikreins 2, 4 or 15 in vivo (4). rhPSA is activated by thermolysin, a zinc protease. The active PSA cleaves several tyrosyl peptide bonds in semenogelins I and II, which are the major gel-forming proteins produced by the seminal vesicles (5). Several inhibitors including serpin A3/ alpha 1-antichymotrypsin (ACT) and alpha 2-macroglobulin are known to form complexes with PSA.

References
  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Ward, A.M. et al. (2001) Ann. Clin. Biochem. 38:633.
  3. Jain, S. et al. (2002) Postgrad. Med. J. 78:646.
  4. LiLja H. (2003) Urology 62:27.
  5. Takayama, T.K. et al. (1997) J. Biol. Chem. 272:21582.
Entrez Gene IDs
354 (Human)
Alternate Names
APS seminin; EC 3.4.21; EC 3.4.21.77; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin

Product Datasheets

Citations for Human Kallikrein 3/PSA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Authors: L Chen, RS Hsi, F Yang, BA Sherer, ML Stoller, SP Ho
    PLoS ONE, 2017;12(11):e0187103.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Cutting edge: Permissive MHC class II allele changes the pattern of antitumor immune response resulting in failure of tumor rejection.
    Authors: Klyushnenkova EN, Kouiavskaia DV, Berard CA, Alexander RB
    J. Immunol., 2009;182(3):1242-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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