Human Kallikrein 7 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Kallikrein 7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, or 15 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 333931
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
    Glu23-His252
    Accession # P49862
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Kallikrein 7 (Catalog # 2624-SE) under non-reducing conditions only
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Kallikrein 7 (Catalog # 2624-SE), see our available Western blot detection antibodies
  • Neutralization
    Measured by its ability to neutralize Recombinant Human Kallikrein 7 (2 µg/mL, Catalog # 2624-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 5.5 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 7

Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length Kallikrein 7 consists of 253 amino acids (aa), with a signal peptide (aa 1-22), short pro peptide (aa 23-29) and mature chain (aa 30-252) (1). Predominantly expressed in the skin, a major physiological function of Kallikrein 7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for Kallikrein 7 is Kallikrein 5, another member of the human tissue Kallikrein family. Along with Kallikrein 14, these three Kallikreins form a proteolytic cascade in the stratum corneum (5).

  • References:
    1. Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
    2. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
    3. Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
    4. Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
    5. Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
  • Entrez Gene IDs:
    5650 (Human); 23993 (Mouse)
  • Alternate Names:
    EC 3.4.21; EC 3.4.21.117; hK7; hSCCE; kallikrein 7 (chymotryptic, stratum corneum); Kallikrein 7; kallikrein-related peptidase 7; KLK7; protease, serine, 6; PRSS6; SCCEkallikrein-7; Serine protease 6; signal protein; Stratum corneum chymotryptic enzyme
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011;131(11):2213-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid.
    Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL
    J. Invest. Dermatol., 2010;130(5):1297-306.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
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