Human Kallikrein 7 Antibody (333931)

Detection of Kallikrein 7 by Western Blot

KLK7 is involved in the A beta degradation activity Inhibition of A beta degradation activity of CCF‐STTG1 cells with MAB2624, a KLK7‐neutralizing antibody. Quantification of the relative remaining A beta 40 and A beta 42 in a mixture of normal culture medium and conditioned medium is shown below the blot (n = 3, mean ± s.e.m., *P < 0.05 by Student's t‐test).Dose‐dependent inhibition of A beta degradation activity of CCF‐STTG1 cells with MAB2624. Quantification of relative remaining A beta 40 in the mixture of normal culture medium and conditioned medium is shown below the blot (n = 3, mean ± s.e.m., **P < 0.01 by Tukey's test).A beta degradation activity in the conditioned medium of COS‐1 cells expressing human KLK7. Quantification of relative remaining A beta 40 in the mixture of normal culture medium and conditioned medium is shown below the blot (n = 3, mean ± s.e.m., ***P < 0.001 by Tukey's test).A beta degradation activity of the recombinant KLK7 protein. Immunoblot analysis of the remaining A beta in the mixture of normal cultured medium and conditioned medium of 7PA2 cells, and recombinant human KLK7 and KLK6 protein is shown. In vitro degradation of preformed A beta fibril by purified MBP‐tagged hKLK7 protein. Amounts of A beta fibrils were measured by thioflavin T fluorescence, and relative fluorescence levels at each time point were shown (n = 3, mean ± s.e.m., *P < 0.05, **P < 0.01 by Tukey's test).Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29311134), licensed under a CC-BY license. Not internally tested by R&D Systems.