Human Klotho  beta Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Klotho beta in direct ELISA and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 885935
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Klotho beta
    Phe53-Leu997
    Accession # Q86Z14
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Klotho  beta by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Klotho  beta Monoclonal Antibody (Catalog # MAB5889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Klotho  beta at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Klotho  beta by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Klotho  beta at approximately 177/170 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Klotho  beta Monoclonal Antibody (Catalog # MAB5889). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Klotho beta
Klotho beta (b-Klotho or KLB) is a 125-135 kDa member of the KL family, Glycosyl Hydrolase 1 superfamily of proteins.  It plays key role in the biology of the endocrine FGFs (FGF-15/19; 21; 23).  KLB appears to serve as a signaling cofactor for select FGFRs, including FGFR-1c, -2c, -3c and FGFR4.  The cofactor is known to constitutively interact with FGFRs, promote FGF binding to their appropriate FGFRs, and to bind directly to FGF-19 and FGF-21.  Notably, FGF-23 appears to utilize a-Klotho/KL for its FGFR signaling cofactor, and FGF-1, while not dependent upon KLB for signaling, can utilize KBL to potentiate certain activities.  KLB is expressed on select cells, including adipocytes and hepatocytes.  Human KLB is a 1044 amino acids (aa) type III transmembrane (TM) protein (i.e.-a type I TM protein lacking a signal sequence).  It contains a 996 aa extracellular region (aa 1-996) and a 27 aa cytoplasmic domain (aa 1018-1044).  The extracellular region contains two glycosyl hydrolase domains (aa 77-508 and 517-967) plus eleven potential N-linked glycosylation sites. Neither hydrolase domain is active as they lack a key Glu residue needed for activity.  Over aa 53-997, human KLB shares 80% aa sequence identity with mouse KLB.  And relative to KL/a-Klotho, human KLB and KL share just 44% aa sequence identity over the entire length of the molecules.     
  • Entrez Gene IDs:
    152831 (Human); 83379 (Mouse); 289625 (Rat)
  • Alternate Names:
    betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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