Detection of Human Ku80/XRCC5 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Ku80/XRCC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Ku80/XRCC5 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ku80 (Lupus Ku autoantigen protein p86; also ATP-dependent DNA helicase 2 subunit 2 and Ku86) is a ubiquitous 80 - 85 kDa nuclear protein that serves as a component of DNA-dependent protein kinase. In particular, it heterodimerizes with Ku70 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku80 is 732 amino acids (aa) in length. It contains a Ku domain (aa 244 - 543) with a DNA binding motif (aa 257 - 268), and a C-terminal DNA-PKcs-binding sequence (aa 720 - 728). Over aa 402 - 565, Ku80 shares 77% aa identity with mouse Ku80.
Lupus Ku Autoantigen Protein 86
Entrez Gene IDs:
7520 (Human); 22596 (Mouse); 363247 (Rat)
80kDa; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC85; CTCBF; EC 3.6.4.-; FLJ39089; G22P2; KARP1; KARP-1; KU80; Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen; Ku86; Ku86DNA repair protein XRCC5; KUB2; NFIV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit; X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5; X-ray repair, complementing defective, repair in Chinese hamster; XRCC5
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