Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 775016
View all Laminin alpha 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Laminin alpha 1
Leu22-Met269
Accession # P25391

Specificity

Detects human Laminin alpha 1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Laminin  alpha 1 Antibody

Detection of Laminin a1 antibody in U2OS Human Cell Line antibody by Flow Cytometry.

Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry.

U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Laminin a1 Monoclonal Antibody (Catalog # MAB4187, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Laminin a1 antibody in U2OS Human Cell Line by Immunocytochemistry (ICC).

Laminin alpha 1 in U2OS Human Cell Line.

Laminin a1 was detected in immersion fixed U2OS human osteosarcoma cell line (positive stain) and A549 human lung carcinoma cell line (negative stain) using Mouse Anti-Human Laminin a1 Monoclonal Antibody (Catalog # MAB4187) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Laminin  alpha 1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line (positive stain) and A549 human lung carcinoma cell line (negative stain)

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: U2OS human cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 5 using MAB4187 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 1

Laminin subunit alpha 1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha, beta, and gamma ) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha 1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha 1 shares 95% and 91% aa sequence identity with canine and mouse alpha 1 chain, respectively.

Alternate Names

LAMA1

Entrez Gene IDs

284217 (Human); 16772 (Mouse); 316758 (Rat)

Gene Symbol

LAMA1

UniProt

P25391

Additional Laminin alpha 1 Products

Product Documents for Human Laminin  alpha 1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Laminin  alpha 1 Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human Laminin  alpha 1 Antibody

Customer Reviews for Human Laminin  alpha 1 Antibody (1)

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  • Human Laminin  alpha 1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: U2OS human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 01/19/2022
    Human Laminin  alpha 1 Antibody MAB4187

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Protocols

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