Lck in Human PBMCs. Lck was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Lck Monoclonal Antibody (Catalog # MAB3704) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of
non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck
contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a
protein kinase domain (aa 251-259). Within aa 2-66, human Lck shares 89%
aa sequence identity with mouse and rat Lck. A short (363 aa) isoform
contains alternate sequence starting at aa 348, while a 539 aa isoform
contains inserted sequence after aa 321. Lck interacts with T cell CD4
and CD8 molecules and plays a pivotal role in regulating T cell
activation. It can be overexpressed in cancers and functions as an
Lymphocyte Protein Tyrosine Kinase
Entrez Gene IDs:
3932 (Human); 16818 (Mouse); 313050 (Rat)
EC 2.7.10; EC 220.127.116.11; Hck-3; Lck; Lcktkr; Leukocyte C-terminal Src kinase; LSK; LSTRA; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16
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