|Detection of Human LEF1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEF1 at approximately 35-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|LEF1 in Neuro‑2A Mouse Cell Line. LEF1 was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
LEF1 (Lymphoid Enhancer-binding Factor 1) is a functionally diverse member of the TCF/LEF family of transcription factors. The name LEF1 was originally applied to the mouse factor, while its human counterpart was named TCF-1 alpha. Since there is a related molecule named TCF-1, to avoid confusion with TCF-1 alpha, both species factors are now called LEF1. Although its predicted MW is 44 kDa, it runs anomalously at 35-55 kDa in SDS-PAGE. LEF1 has restricted expression in adult, being limited to T cells, thymocytes, pre-B cells and NK cells. LEF1 demonstrates sequence-specific (CCTTTG[T/A][T/A]) DNA binding and directs beta -catenin to Wnt‑responsive genes. Either transcriptional activation or repression may occur on LEF1 target genes, depending upon 1) the cofactors recruited to the LEF1: beta ‑catenin complex, and 2) the phosphorylation state of LEF1. Human LEF1 is 399 amino acids (aa) in length. It contains one DNA-binding HMG-box (aa 298‑369), three potential phosphorylation sites, and an N-terminal SUMOylation site at Lys27. There are at least five isoform variants. One utilizes an alternative start site at Met116, a second contains a 47 aa substitution for aa 283‑299, and a third combines the previous two variations. A fourth variant shows a deletion of aa 214‑241, while a fifth variant combines the afore mentioned deletion of aa 214-241 with a 25 aa substitution for aa 390‑399. Over aa 120‑280, human LEF1 shares 97% aa sequence identity with mouse LEF1.
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