Human Lefty-A Long and Short Isoforms Antibody

Catalog # Availability Size / Price Qty
MAB7461
MAB7461-SP
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Human Lefty-A Long and Short Isoforms Antibody Summary

Species Reactivity
Human
Specificity
Detects both long and short forms of recombinant human (rh) Lefty-A in direct ELISAs and Western blots. No cross‑reactivity with rhArtemin, recombinant mouse (rm) Artemin, recombinant drosophila DPP, rhGDNF, rhLAP, recombinant rat MIS, rhNeurturin, rhTGF-alpha, rhTGF-beta 1, rhTGF‑ beta 1.2, or rmLefty-1. The cross‑reactivity of this antibody with rhLefty-B (96% amino acid sequence homology) was not determined.
Source
Monoclonal Mouse IgG2B Clone # 182525
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lefty-A
Phe78-Pro366
Accession # O00292.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Lefty-A (Catalog # 746-LF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lefty-A

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial Bleeding-Associated Factor. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and -B are more similar to each other (96%) than to either Lefty-1 or -2 in the mouse (81‑82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, Lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of Lefty are larger than mature forms of other TGF‑ beta ‑related proteins. Post-translational cleavage of a propeptide occurs at two different sites resulting in long (aa 78‑366) and short (aa 136‑366) forms of mature human Lefty-A. Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of Lefty on the left side is well conserved. Furthermore its function in patterning left‑right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

References
  1. Meno, C. et al. (1996) Nature 381:151.
  2. Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712.
  3. Schier, A.F. and M.M. Shen, (1999) Nature 403:385.
  4. Branford, W.W. et al. (2000) Dev. Biol. 223:291.
  5. Tabibzadeh, S. et al. (2000) J. Clin. Endocr. Metab. 85:2526.
  6. Ulloa, L. et al. (2001) J. Biol. Chem. 276:21387.
Entrez Gene IDs
7044 (Human)
Alternate Names
EBAFTGF-beta-4; endometrial bleeding associated factor; Endometrial bleeding-associated factor; Lefta; LEFTAProtein lefty-A; left-right determination factor 2; Left-right determination factor A; LEFTY2; LeftyA; Lefty-A; LEFTYATransforming growth factor beta-4; MGC46222; Protein lefty-2; TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily)

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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