Human Leptin/OB Antibody

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Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody.
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Product Details
Citations (11)
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Reviews (1)

Human Leptin/OB Antibody Summary

Species Reactivity
Captures human Leptin/OB in sandwich ELISAs. In this format, this antibody shows less than 0.1% cross-reactivity with recombinant mouse (rm) Leptin/OB and rrLeptin/OB.
Monoclonal Mouse IgG1 Clone # 44802
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Leptin/OB
Accession # P41159
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Complete Your Research


Recommended Concentration

Human Leptin/OB Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Leptin/OB Biotinylated Antibody (Catalog # BAM398)

Standard: Recombinant Human Leptin Protein, CF (Catalog # 398-LP)

Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.4-1.6 µg/mL in the presence of 7.5 ng/mL Recombinant Human Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. View Larger

Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin/OB Monoclonal Antibody (Catalog # MAB398). The ND50 is typically 0.4-1.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

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Citations for Human Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Appetite Control across the Lifecourse: The Acute Impact of Breakfast Drink Quantity and Protein Content. The Full4Health Project
    Authors: DR Crabtree, W Buosi, CL Fyfe, GW Horgan, Y Manios, O Androutsos, A Giannopoul, G Finlayson, K Beaulieu, CL Meek, JJ Holst, KV Van Norren, JG Mercer, AM Johnstone
    Nutrients, 2020;12(12):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Capture)
  2. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014;111(6):2110-5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Microfluidic Assay
  3. Regulatory T cell proliferative potential is impaired in human autoimmune disease.
    Authors: Carbone F, De Rosa V, Carrieri P, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G
    Nat Med, 2014;20(1):69-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  6. Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
    Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
    Metab. Clin. Exp., 2009;58(7):946-53.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  7. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  8. Leptin, blood pressure, and aerobic capacity in women.
    Authors: Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG
    Am. J. Hypertens., 2008;21(11):1245-50.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  9. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007;7(0):5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  10. Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    J. Assist. Reprod. Genet., 2005;22(6):257-64.
    Species: Human
    Sample Types: Follicular Fluid
    Applications: ELISA Development
  11. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002;13(2):225-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development


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Human Leptin/OB Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human