Human Lgr6 Antibody

Catalog # Availability Size / Price Qty
MAB84581-100
MAB84581-SP
Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry.
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Human Lgr6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lgr6 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 918726
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Lgr6
Met1-Val967
Accession # Q9HBX8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6. Binding is completely blocked by 1 μg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581).
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Lgr6 antibody in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP antibody by Flow Cytometry. View Larger

Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Lgr6 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

Lgr6 in HEK293 Human Cell Line. Lgr6 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6 using Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Blockade of Receptor-ligand Interaction R-Spondin 4 Binding to Lgr6-transfected HEK293 Human Cell Line is Blocked by Human Lgr6 Antibody. View Larger

R-Spondin 4 Binding to Lgr6-transfected HEK293 Human Cell Line is Blocked by Human Lgr6 Antibody. In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6 (dark orange filled histogram). Binding is completely blocked (light orange filled histogram) by 1 µg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581). Mouse IgG2A Isotype Control (Catalog # MAB003) at 1 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr6

Lgr6 is a glycoprotein hormone receptor phosphorylated upon DNA damage, probably by ATM or ATR. It is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. Lgr6 is a high affinity receptor for R-Spondins 1-3 and potentially functions as a tumor suppressor despite its positive effect on Wnt/ beta -Catenin signaling.

Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 6
Entrez Gene IDs
59352 (Human); 329252 (Mouse); 498233 (Rat)
Alternate Names
FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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