Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, Flow Cytometry, Immunocytochemistry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 918726
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Lgr6
Met1-Val967
Accession # Q9HBX8
Met1-Val967
Accession # Q9HBX8
Specificity
Detects human Lgr6 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Lgr6 Antibody
Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Lgr6 in HEK293 Human Cell Line.
Lgr6 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6 using Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
R-Spondin 4 Binding to Lgr6-transfected HEK293 Human Cell Line is Blocked by Human Lgr6 Antibody.
In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6 (dark orange filled histogram). Binding is completely blocked (light orange filled histogram) by 1 µg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581). Mouse IgG2A Isotype Control (Catalog # MAB003) at 1 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).Applications for Human Lgr6 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6. Binding is completely blocked by 1 μg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581).
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Lgr6
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 6
Alternate Names
FLJ14471, gonadotropin receptor, GPCR, leucine-rich repeat containing G protein-coupled receptor 6, leucine-rich repeat-containing G protein-coupled receptor 6, leucine-rich repeat-containing G-protein coupled receptor 6, VTS20631
Gene Symbol
LGR6
UniProt
Additional Lgr6 Products
Product Documents for Human Lgr6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Lgr6 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
