Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse LIF is observed. Neutralizes the biological activity of recombinant human LIF and will also neutralize the biological activity of recombinant mouse LIF at a 50-fold higher IgG concentration.
Polyclonal Goat IgG
Protein A or G purified
E. coli-derived recombinant human LIF Ser23-Phe202 Accession # P15018
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human LIF
Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.5 ng/mL Recombinant Human LIF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND50 is typically 0.1‑0.4 µg/mL.
Preparation and Storage
Reconstitute at 1 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LIF (Leukemia inhibitory factor; also Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.
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