Human LIGHT/TNFSF14 Antibody
Human LIGHT/TNFSF14 Antibody Summary
Accession # O43557
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6642).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human LIGHT/TNFSF14 DuoSet ELISA Kit (Catalog # DY664) for convenient development of a sandwich ELISA or the Human LIGHT/TNFSF14 Quantikine ELISA Kit (Catalog # DLIT00) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
LIGHT/TNFSF14 in Human Spleen. LIGHT/TNFSF14 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by LIGHT/TNFSF14 and Neutral-ization by Human LIGHT/ TNFSF14 Antibody. Recombinant Human LIGHT/ TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human LIGHT/ TNFSF14 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LIGHT/ TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664). The ND50 is typically 5-20 ng/mL.
Human LIGHT/TNFSF14 ELISA Standard Curve. Recombinant Human LIGHT/TNFSF14 protein was serially diluted 2-fold and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (MAB6642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.
- Mauri, D.N. et al. (1998) Immunity 8:21.
- Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
- Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
- Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Citations for Human LIGHT/TNFSF14 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
Sample Types: Whole Cells
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
Sample Types: Whole Cells
VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
Nat. Neurosci., 2006-02-05;9(3):340-8.
Sample Types: Whole Tissue
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