Detects human LIGHT/TNFSF14 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) APRIL is observed and less than 1% cross-reactivity with rhFas Ligand, rhTRAIL, and rhTNF-alpha is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14 Asp74-Val240 Accession # O43557
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI)
Measured by its ability to neutralize LIGHT/TNFSF14-induced proliferation in the HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically 5-20 ng/mL in the presence of 10 ng/mL Recombinant Human LIGHT/TNFSF14.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
LIGHT/TNFSF14 in Human Spleen. LIGHT/TNFSF14 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by LIGHT/TNFSF14 and Neutralization by Human LIGHT/ TNFSF14 Antibody.
Recombinant Human LIGHT/ TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human LIGHT/ TNFSF14 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LIGHT/ TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664). The ND50 is typically 5‑20 ng/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.
Mauri, D.N. et al. (1998) Immunity 8:21.
Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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