Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 561203
View all LPAR4/LPA4 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human LPAR4/LPA4
Accession # Q99677

Specificity

Detects human LPAR4/LPA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human LPAR4/LPA4 Antibody

Detection of LPAR4 antibody in HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP antibody by Flow Cytometry.

Detection of LPAR4 in HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) Human LPAR4 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human LPAR4 Monoclonal Antibody (Catalog # MAB10217) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LPAR4/LPA4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LPAR4/LPA4

Lysophosphatidic acid receptor 4, also known as LPA4, P2Y purinoceptor 9 (P2RY9) or GPR23, is a protein that in humans is encoded by the LPAR4 gene. LPA4 is a G protein-coupled receptor (GPCR) that binds the lipid signaling molecule lysophosphatidic acid (LPA) and mediates diverse cellular activities. Most LPA receptors share similarities with members of the S1P1/Edg family. GPA4, originally named P2Y9/GPR23, has been described as a fourth LPA receptor, LPA4, that together with LPA5 and LPA6 form a subfamily of LPA receptors structurally distant from the Edg family.  

Long Name

Lysophosphatidic Acid Receptor 4

Alternate Names

GPCR23, GPR23, P2RY9, P2Y9

Entrez Gene IDs

2846 (Human); 78134 (Mouse)

Gene Symbol

LPAR4

UniProt

Q99677

Additional LPAR4/LPA4 Products

Product Documents for Human LPAR4/LPA4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LPAR4/LPA4 Antibody

For research use only

Related Research Areas

Bioactive Lipids and Receptors

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