Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1053415
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
Met1-Gly138
Accession # Q17RY6
Met1-Gly138
Accession # Q17RY6
Specificity
Detects human Ly6K in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Ly6K Antibody
Detection of Ly6K in HeLa (positive) and K562 (negative) cells.
Ly6K was detected in immersion fixed HeLa (positive) and K562 (negative) cells using Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Ly6K by Western Blot.
Western blot shows lysates of human Testes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ly6K at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human Ly6K Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa (positive) and K562 (negative) cells
Sample: Immersion fixed HeLa (positive) and K562 (negative) cells
Western Blot
2 µg/mL
Sample:
Sample:
| Human Testes |
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Ly6K
Long Name
Lymphocyte Antigen 6 Complex Locus K
Alternate Names
CO16, CT97
Gene Symbol
LY6K
UniProt
Additional Ly6K Products
Product Documents for Human Ly6K Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Ly6K Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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