Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 135105
View all Lymphotoxin beta/TNFSF3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin  alpha / beta

Specificity

Detects human Lymphotoxin alpha beta heterotrimers in direct ELISAs and Western blots. This antibody detects an epitope in the Lymphotoxin  beta subunit. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Lymphotoxin  alpha, rhAPRIL, rhBAFF, rhTNF‑ alpha, rhTWEAK, rhEDA‑A2, rhEDA, rhTRAIL, rhVEGI, rhTRANCE, rhFas Ligand, or rhOX40 Ligand.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Lymphotoxin  beta /TNFSF3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human Lymphotoxin  beta /TNFSF3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin beta/TNFSF3

Lymphotoxin beta is a type II transmembrane protein member of the TNF ligand superfamily and is now designated TNFSF3. Lymphotoxin beta associates with Lymphotoxin alpha to form two types of heterotrimers, Lymphotoxin alpha 1 beta 2 and Lymphotoxin  alpha 2 beta 1. Lymphotoxin beta is expressed by activated CD4 T cells, unpolarized IL‑2‑secreting effector T cells, and Th1 effector cells.

Long Name

Lymphotoxin beta

Alternate Names

LTB, TNFSF3

Entrez Gene IDs

4050 (Human); 102135554 (Cynomolgus Monkey)

Gene Symbol

LTB

Additional Lymphotoxin beta/TNFSF3 Products

Product Documents for Human Lymphotoxin  beta /TNFSF3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lymphotoxin  beta /TNFSF3 Antibody

For research use only

Citations for Human Lymphotoxin  beta /TNFSF3 Antibody

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