Human MAP2 Antibody

Catalog # Availability Size / Price Qty
MAB8304
MAB8304-SP
MAP2 in Human Embryonic Stem Cells.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human MAP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MAP2 in direct ELISAs.
Source
Monoclonal Mouse IgG3 Clone # 885232
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MAP2
Gly1689-Lys1824
Accession # P11137
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
0.5-25 µg/mL
See below
Immunocytochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MAP2 antibody in Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

MAP2 in Human Embryonic Stem Cells. MAP2 was detected in immersion fixed human embryonic stem cells/neurospheres using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry MAP2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

MAP2 in Human Brain. MAP2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurofibrillary tangles and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAP2

MAP2 (Microtubule-associated protein 2) is a 1827 amino acid (aa) cytoskeletal associated protein.  Human MAP2 shares 84% and 79% aa identity with mouse and rat MAP2, respectively.  Multiple splice forms exist, resulting in 4 distinct isoforms.  MAP2 functions in microtubule polymerization and stabilization in both normal and malignant cell types.  It has been demonstrated to play a critical role in neurite outgrowth and loss of MAP2 function in neurons may lead to neural degeneration.

Long Name
Microtubule-associated Protein 2
Entrez Gene IDs
4133 (Human); 17756 (Mouse)
Alternate Names
DKFZp686I2148; MAP2; MAP-2; MAP2A; MAP2B; MAP2C; Microtubule Associated Protein 2; microtubule-associated protein 2; MTAP2

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Citations for Human MAP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons
    Authors: Kuroda, T;Yasuda, S;Matsuyama, S;Miura, T;Sawada, R;Matsuyama, A;Yamamoto, Y;Morioka, MS;Kawaji, H;Kasukawa, T;Itoh, M;Akutsu, H;Kawai, J;Sato, Y;
    Scientific reports
    Species: Human
    Sample Types: Transduced Whole Cells, Whole Cells
    Applications: ICC
  2. Cell Specificity of Manganese-enhanced MRI Signal in the Cerebellum
    Authors: Rallapalli, H;Bayin, NS;Goldman, H;Maric, D;Nieman, BJ;Koretsky, AP;Joyner, AL;Turnbull, DH;
    NeuroImage
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. CXCL13 expressed on inflamed cerebral blood vessels recruit IL-21 producing TFH cells to damage neurons following stroke
    Authors: Aditya Rayasam, Julie A. Kijak, Lee Kissel, Yun Hwa Choi, Taehee Kim, Martin Hsu et al.
    Journal of Neuroinflammation
  4. NRSF/REST lies at the intersection between epigenetic regulation, miRNA-mediated gene control and neurodevelopmental pathways associated with Intellectual disability (ID) and Schizophrenia
    Authors: Mouhamed Alsaqati, Brittany A. Davis, Jamie Wood, Megan M. Jones, Lora Jones, Aishah Westwood et al.
    Translational Psychiatry
  5. Evidence of shared transcriptomic dysregulation of HNRNPU-related disorder between human organoids and embryonic mice
    Authors: Andrew K. Ressler, Gabriela L.A. Sampaio, Sarah A. Dugger, Tamar Sapir, Daniel Krizay, Michael J. Boland et al.
    iScience

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Reviews for Human MAP2 Antibody

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Human MAP2 Antibody
By Anonymous on 12/07/2021
Application: IHC Sample Tested: Brain tissue Species: Human