Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunocytochemistry, COMET

Cited:

Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 885232
View all MAP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MAP2
Gly1689-Lys1824
Accession # P11137

Specificity

Detects human MAP2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human MAP2 Antibody

Detection of MAP2 in Human Brain Cortex via seqIF™ staining on COMET™

MAP2 was detected in immersion fixed paraffin-embedded sections of human Brain Cortex using Mouse Anti-Human MAP2, Monoclonal Antibody (Catalog # MAB8304) at 20ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​
MAP2 antibody in Human Embryonic Stem Cells by Immunocytochemistry (ICC).

MAP2 in Human Embryonic Stem Cells.

MAP2 was detected in immersion fixed human embryonic stem cells/neurospheres using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
MAP2 antibody in Human Brain by Immunohistochemistry (IHC-P).

MAP2 in Human Brain.

MAP2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurofibrillary tangles and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MAP2 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

0.5-25 µg/mL
Sample: Immersion fixed human embryonic stem cells/neurospheres

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain

Multiplex Immunofluorescence

20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Brain Cortex

Reviewed Applications

Read 1 review rated 5 using MAB8304 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAP2

MAP2 (Microtubule-associated protein 2) is a 1827 amino acid (aa) cytoskeletal associated protein.  Human MAP2 shares 84% and 79% aa identity with mouse and rat MAP2, respectively.  Multiple splice forms exist, resulting in 4 distinct isoforms.  MAP2 functions in microtubule polymerization and stabilization in both normal and malignant cell types.  It has been demonstrated to play a critical role in neurite outgrowth and loss of MAP2 function in neurons may lead to neural degeneration.

Long Name

Microtubule-associated Protein 2

Alternate Names

DKFZp686I2148, MAP-2, MAP2A, MAP2B, MAP2C, Microtubule Associated Protein 2, microtubule-associated protein 2, MTAP2

Entrez Gene IDs

4133 (Human); 17756 (Mouse)

Gene Symbol

MAP2

UniProt

P11137

Additional MAP2 Products

Product Documents for Human MAP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MAP2 Antibody

For research use only

Citations for Human MAP2 Antibody

Customer Reviews for Human MAP2 Antibody (1)

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  • Human MAP2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 12/07/2021
    Human MAP2 Antibody MAB8304

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Protocols

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FAQs

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