MAP2 in Human Embryonic Stem Cells. MAP2 was detected in immersion fixed human embryonic stem cells/neurospheres using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
MAP2 in Human Brain. MAP2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurofibrillary tangles and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MAP2 (Microtubule-associated protein 2) is a 1827 amino acid (aa) cytoskeletal associated protein. Human MAP2 shares 84% and 79% aa identity with mouse and rat MAP2, respectively. Multiple splice forms exist, resulting in 4 distinct isoforms. MAP2 functions in microtubule polymerization and stabilization in both normal and malignant cell types. It has been demonstrated to play a critical role in neurite outgrowth and loss of MAP2 function in neurons may lead to neural degeneration.
Microtubule-associated Protein 2
Entrez Gene IDs:
4133 (Human); 17756 (Mouse)
DKFZp686I2148; MAP-2; MAP2A; MAP2B; MAP2C; microtubule-associated protein 2
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