Human Matrilin-2 Antibody

  
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  • Species Reactivity
    Human
  • Specificity
    Detects human Matrilin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant mouse (rm) Matrilin-2 is observed and approximately 15% cross-reactivity with rmMatrilin-3 and recombinant human Matrilin-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Matrilin-2 short isoform
    Arg24-Arg937
    Accession # AAH10444
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Matrilin‑2 (Catalog # 3044-MN)
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed WS-1 human fetal skin skin fibroblast cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Matrilin-2

Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix proteins (1‑4). The human Matrilin-2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C-terminal coiled-coil domain (5, 6). Alternate splicing generates Isoform 2 (with an 18 aa deletion near the C-terminus), Isoform 3 (with a deletion of the fourth EGF-like repeat), and Isoform 4 (with a deletion of the first VWA and first EGF-like repeat). Human Matrilin-2 shares 87% and 84% aa sequence identity with mouse and canine Matrilin-2, respectively, and 27%, 22%, and 33% aa sequence identity with human Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled-coil domain (4, 7, 8, 9). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 7, 8) but has not been detected in heterotrimers containing Matrilin-3 (8). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrillin-2, and Laminin-1/Nidogen-1 complexes (7). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (10).

  • References:
    1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
    2. Deak, F. et al. (1999) Matrix Biol. 18:55.
    3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
    4. Piecha, D. et al. (1999) J. Biol. Chem. 274:13353.
    5. Muratoglu, S. et al. (2000) Cytogenet. Cell Genet. 90:323.
    6. Deak, F. et al. (1997) J. Biol. Chem. 272:9268.
    7. Piecha, D. et al. (2002) Biochem. J. 367:715.
    8. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
    9. Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205.
    10. Mates, L. et al. (2004) Matrix Biol. 23:195.
  • Entrez Gene IDs:
    4147 (Human)
  • Alternate Names:
    MATN2; matrilin 2; Matrilin2; Matrilin-2
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