Human MEK2 Antibody

Name: Human MEK2 Antibody
Brand: R&D Systems
SKU: AF2855
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # AF2855

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Product Specifications
Scientific Data
Applications
Preparation & Storage
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all MEK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MEK2
Met1-Val400
Accession # P36507

Specificity

Detects human MEK2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MEK2 Antibody

Detection of Human MEK2 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human MEK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2855) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For add-itional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (10 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MEK2 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: MEK2

Mitogen-activated protein kinase kinase 2 (MAP2K2), also known as MEK2 and MKK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dual-specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK2 by Raf occurs through phosphorylation at S222 and S226.