Human MESDC2 Antibody

Catalog # Availability Size / Price Qty
MAB5577
MAB5577-SP
Detection of Human MESDC2 by Western Blot.
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Human MESDC2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MESDC2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse MESDC2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 513802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MESDC2
Ala34-Leu234
Accession # Q14696
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human MESDC2 by Western Blot. View Larger

Detection of Human MESDC2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human MESDC2 Monoclonal Antibody (Catalog # MAB5577) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MESDC2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESDC2

Mesoderm development candidate gene 2 (MESDC2), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis. MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression. Within aa 34‑234, human MESDC2 shares 87% aa sequence identity with mouse and rat MESDC2, respectively.

Long Name
Mesoderm Development Candidate 2
Entrez Gene IDs
23184 (Human)
Alternate Names
BOCA; KIAA0081Renal carcinoma antigen NY-REN-61; MESDC2; MESDLDLR chaperone MESD; mesoderm development candidate 2Mesoderm development protein

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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