Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1075034
View all METTL3 Primary Antibodies »

Product Specifications

Immunogen

METTL3 containing Synthetic Peptide
Accession # Q86U44

Specificity

Detects human METTL3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human METTL3 Antibody

Detection of METTL3 in Human Lung Cancer.

METTL3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human METTL3 Monoclonal Antibody (Catalog # MAB11514) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of METTL3 in HepG2 Human Cell Line.

METTL3 was detected in fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human METTL3 Monoclonal Antibody (Catalog # MAB11514) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human METTL3 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: METTL3

METTL3 is a 70 kDa subunit and is the sole catalytic subunit in the methyltransferase complex which catalyzes N6-methyladenosine.  N6-methlyladenosine is the most abundant mRNA modification.  The full-length METTL3 protein consists of 580 amino acids. METTL3 plays a key role in a variety of cancers and expression is dysregulated via different mechanisms.  It plays a critical role in tumorigenesis, tumor growth, metastasis, metabolic reprogramming, immune cell infiltration, and tumor drug resistance. METTL3 can be an oncogene through depositing m6A modifications on critical transcripts.  In some cases, it can also be a tumor suppressor possibly by promoting cell cycle arrest in the G1 phase.

References

  1. Zeng C, Huang W, Li Y, Weng H. Roles of METTL3 in cancer: mechanisms and therapeutic targeting. J Hematol Oncol. 2020 Aug 27;13(1):117. doi: 10.1186/s13045-020-00951-w. PMID: 32854717; PMCID: PMC7457244. 
  2. Jin Q, Qu H, Quan C. New insights into the regulation of METTL3 and its role in tumors. Cell Commun Signal. 2023 Nov 23;21(1):334. doi: 10.1186/s12964-023-01360-5. PMID: 37996892; PMCID: PMC10732098.

Long Name

Methyltransferase like 3

Alternate Names

adoMet-binding subunit of the human mRNA (N6-adeno, hMETTL3, IME4, mRNA m(6)A methyltransferase, MT-A70, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, Spo8

Entrez Gene IDs

56339 (Human); 56335 (Mouse); 361035 (Rat)

Gene Symbol

Q86U44

UniProt

Q86U44 (Human)

Additional METTL3 Products

Product Documents for Human METTL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human METTL3 Antibody

For research use only

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Protocols

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