Human mGluR3 Antibody Summary
Accession # Q14832
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of MGLUR3 in HEK293 Human Cell Line Transfected with Human MGLUR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human MGLUR3 and eGFP was stained with (A) Mouse Anti-Human MGLUR3 Monoclonal Antibody (Catalog # MAB4668) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
mGluR3 in Human Hypothalamus Tissue. mGluR3 was detected in immersion fixed paraffin-embedded sections of human hypothalamus tissue using Mouse Anti-Human mGluR3 Monoclonal Antibody (Catalog # MAB4668) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human mGluR3 Antibody
There are currently no reviews for this product. Be the first to review Human mGluR3 Antibody and earn rewards!
Have you used Human mGluR3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image