Human Mind Bomb 2/MIB2 Antibody

Catalog # Availability Size / Price Qty
MAB7289
MAB7289-SP
Mind Bomb 2/MIB2 in U266 Human Cell Line.
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Human Mind Bomb 2/MIB2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mind Bomb 2/MIB2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 739501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Mind Bomb 2/MIB2 antibody in U266 Human Cell Line by Immunocytochemistry (ICC). View Larger

Mind Bomb 2/MIB2 in U266 Human Cell Line. Mind Bomb 2/MIB2 was detected in immersion fixed U266 human myeloma cell line using Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB7289) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mind Bomb 2/MIB2