Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 739501
View all Mind Bomb 2/MIB2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9

Specificity

Detects human Mind Bomb 2/MIB2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mind Bomb 2/MIB2 Antibody

Mind Bomb 2/MIB2 antibody in U266 Human Cell Line by Immunocytochemistry (ICC).

Mind Bomb 2/MIB2 in U266 Human Cell Line.

Mind Bomb 2/MIB2 was detected in immersion fixed U266 human myeloma cell line using Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB7289) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Mind Bomb 2/MIB2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U266 human myeloma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mind Bomb 2/MIB2

Long Name

Mindbomb Homolog 2

Alternate Names

MIB2, Mind Bomb-2, Novelzin, SKD, Skeletrophin, ZZANK1, ZZZ5

Entrez Gene IDs

142678 (Human); 76580 (Mouse)

Gene Symbol

MIB2

UniProt

Q96AX9 (Human)

Additional Mind Bomb 2/MIB2 Products

Product Documents for Human Mind Bomb 2/MIB2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Mind Bomb 2/MIB2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
E3 Ubiquitin Ligases

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