Human MKK7 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MKK7 in Western blots. Does not cross-react with other recombinant human mitogen-activated protein kinase kinases.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MKK7
    Met1-Arg419
    Accession # O14733
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human tonsil and placenta
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MKK7 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human MKK7 by Western Blot. Western blot shows recombinant human MEKs and recombinant human MKKs Catalog#s??? (??? ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKK7
MAP Kinase Kinase 7 (MKK7), also known as MAP2K7, is a member of the dual-specificity MAP kinase kinase family with a predicted molecular weight of approximately 47.5 kDa. It is widely expressed in mammalian tissue and localizes to both the cytoplasm and the nucleus. The MKK7 protein contains three N-terminal D-sites, a central catalytic domain, and a C-terminal DVD domain. MAP kinase kinase kinases activate MKK7 via binding of the DVD domain and phosphorylation of a serine and threonine residue within the activation loop of the catalytic domain. MKK7 then utilizes its D-sites to bind and activate the JNK family of MAP kinases via phosphorylation. The genetic inactivation of MKK7 in mice results in embryonic lethality, indicating that MKK7 is an essential gene. Accordingly, MKK7 appears to be involved in the regulation of diverse physiological processes, including axon elongation, the circadian clock, embryonic stem cell differentiation, and liver regeneration. MKK7 function may also be important for the prevention of certain diseases. It has been shown to inhibit lung and mammary cancer via JNK1- and JNK2-dependent p53 stabilization. Additionally, reduced MKK7 function has been linked to the development of schizophrenia.
  • Long Name:
    Mitogen-activated Protein Kinase Kinase 7
  • Entrez Gene IDs:
    5609 (Human); 26400 (Mouse); 363855 (Rat)
  • Alternate Names:
    c-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; EC 2.7.12.2; JNK kinase 2; JNK-activating kinase 2; JNKK 2; JNKK2; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK7; MEK 7; MEK7; mitogen-activated protein kinase kinase 7; MKE7; MKK7; MKK7MAPKK 7; PRKMK7
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