Human MMP-13 Antibody

Catalog # Availability Size / Price Qty
MMP‑13 in MDA‑MB‑231 Human Cell Line.
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Product Details
Citations (13)
Supplemental Products
Reviews (5)

Human MMP-13 Antibody Summary

Species Reactivity
Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Monoclonal Mouse IgG1 Clone # 87512
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Accession # P45452
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human MMP‑13 Western Blot Standard (Catalog # WBC020)
8-25 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies
Immunoaffinity Purification
Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human MMP-13 (Catalog # 511-MM) from conditioned media.
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MMP-13 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑13 in MDA‑MB‑231 Human Cell Line. MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP‑13 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑13 in Human Ovarian Cancer Tissue. MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-13

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Long Name
Matrix Metalloproteinase 13
Entrez Gene IDs
4322 (Human)
Alternate Names
CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC; EC; EC; EC; EC; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13

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Citations for Human MMP-13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. A 3D Collagen-Based Bioprinted Model to Study Osteosarcoma Invasiveness and Drug Response
    Authors: E Pellegrini, G Desando, M Petretta, A Cellamare, C Cristalli, M Pasello, MC Manara, B Grigolo, K Scotlandi
    Polymers, 2022;14(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker
    Authors: M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström
    Cancers, 2022;14(14):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Evidence for osteocyte-mediated bone-matrix degradation associated with periprosthetic joint infection (PJI)
    Authors: RT Ormsby, AR Zelmer, D Yang, NJ Gunn, Y Starczak, SP Kidd, R Nelson, LB Solomon, GJ Atkins
    European cells & materials, 2021;41(0):264-280.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model
    Authors: SK Kim, SD Jang, H Kim, S Chung, JK Park, HJ Kuh
    Cancers (Basel), 2020;12(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  5. Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
    Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
    Bone Rep, 2020;13(0):100715.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  6. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
    Authors: Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
    Nat Commun, 2014;5(0):4255.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Toll-like receptor 9 ligands enhance mesenchymal stem cell invasion and expression of matrix metalloprotease-13.
    Authors: Nurmenniemi S, Kuvaja P, Lehtonen S
    Exp. Cell Res., 2010;316(16):2676-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Ecto-5'-nucleotidase (CD73) -mediated extracellular adenosine production plays a critical role in hepatic fibrosis.
    Authors: Peng Z, Fernandez P, Wilder T, Yee H, Chiriboga L, Chan ES, Cronstein BN
    FASEB J., 2008;22(7):2263-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
    Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
    Int. J. Cancer, 2008;122(12):2805-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Basic fibroblast growth factor stimulates matrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cdelta pathways in human adult articular chondrocytes.
    Authors: Im HJ, Muddasani P, Natarajan V, Schmid TM, Block JA, Davis F, van Wijnen AJ, Loeser RF
    J. Biol. Chem., 2007;282(15):11110-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
    Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM
    J. Cell. Physiol., 2007;210(2):417-27.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. The inhibition of subchondral bone resorption in the early phase of experimental dog osteoarthritis by licofelone is associated with a reduction in the synthesis of MMP-13 and cathepsin K.
    Authors: Pelletier JP, Boileau C, Brunet J, Boily M, Lajeunesse D, Reboul P, Laufer S, Martel-Pelletier J
    Bone, 2004;34(3):527-38.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P


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Reviews for Human MMP-13 Antibody

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Human MMP-13 Antibody
By Mozhdeh Sojoodi on 02/07/2022
Application: ICC/IF Sample Tested: Liver tissue Species: Mouse

1:100 dilution
Citrate buffer Ag retrieval
Overnight incubation of the Ab, RT

Human MMP-13 Antibody
By Anonymous on 12/06/2021
Application: IHC Sample Tested: Prostate cancer tissue Species: Human

Human MMP-13 Antibody
By Anonymous on 10/14/2021
Application: IHC Sample Tested: Joint tissue Species: Human

Human MMP-13 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Human

Human MMP-13 Antibody
By Anonymous on 11/10/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The antibody AF511 was used as the detection antibody with this antibody as the capture in an ELISA targeting MMP-13. The immunoassay was used to measure MMP-13 levels in human plasma samples.