Human MMP-13 Antibody

Catalog # Availability Size / Price Qty
MAB511-SP
MAB511-500
MAB511-100
Human MMP-13 Antibody in Data
2 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)

Human MMP-13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Source
Monoclonal Mouse IgG1 Clone # 87512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑13 Western Blot Standard (Catalog # WBC020)
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies
Immunoaffinity Purification
Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human MMP-13 (Catalog # 511-MM) from conditioned media.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry MMP-13 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑13 in MDA‑MB‑231 Human Cell Line. MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP‑13 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑13 in Human Ovarian Cancer Tissue. MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-13

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References
  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Long Name
Matrix Metalloproteinase 13
Entrez Gene IDs
4322 (Human)
Alternate Names
CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.35; EC 3.4.24.65; EC 3.4.24.7; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13

Product Datasheets

Citations for Human MMP-13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model
    Authors: SK Kim, SD Jang, H Kim, S Chung, JK Park, HJ Kuh
    Cancers (Basel), 2020;12(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  2. Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
    Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
    Bone Rep, 2020;13(0):100715.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  3. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
    Authors: Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
    Nat Commun, 2014;5(0):4255.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Toll-like receptor 9 ligands enhance mesenchymal stem cell invasion and expression of matrix metalloprotease-13.
    Authors: Nurmenniemi S, Kuvaja P, Lehtonen S
    Exp. Cell Res., 2010;316(16):2676-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Ecto-5'-nucleotidase (CD73) -mediated extracellular adenosine production plays a critical role in hepatic fibrosis.
    Authors: Peng Z, Fernandez P, Wilder T, Yee H, Chiriboga L, Chan ES, Cronstein BN
    FASEB J., 2008;22(7):2263-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
    Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
    Int. J. Cancer, 2008;122(12):2805-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Basic fibroblast growth factor stimulates matrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cdelta pathways in human adult articular chondrocytes.
    Authors: Im HJ, Muddasani P, Natarajan V, Schmid TM, Block JA, Davis F, van Wijnen AJ, Loeser RF
    J. Biol. Chem., 2007;282(15):11110-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
    Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM
    J. Cell. Physiol., 2007;210(2):417-27.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. The inhibition of subchondral bone resorption in the early phase of experimental dog osteoarthritis by licofelone is associated with a reduction in the synthesis of MMP-13 and cathepsin K.
    Authors: Pelletier JP, Boileau C, Brunet J, Boily M, Lajeunesse D, Reboul P, Laufer S, Martel-Pelletier J
    Bone, 2004;34(3):527-38.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MMP-13 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human MMP-13 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human MMP-13 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Human

Human MMP-13 Antibody
By Anonymous on 11/10/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The antibody AF511 was used as the detection antibody with this antibody as the capture in an ELISA targeting MMP-13. The immunoassay was used to measure MMP-13 levels in human plasma samples.