Human MMP-13 Antibody
Human MMP-13 Antibody Summary
Leu20-Cys471
Accession # P45452
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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MMP‑13 in MDA‑MB‑231 Human Cell Line. MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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MMP‑13 in Human Ovarian Cancer Tissue. MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-13
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
- Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Product Datasheets
Citations for Human MMP-13 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A 3D Collagen-Based Bioprinted Model to Study Osteosarcoma Invasiveness and Drug Response
Authors: E Pellegrini, G Desando, M Petretta, A Cellamare, C Cristalli, M Pasello, MC Manara, B Grigolo, K Scotlandi
Polymers, 2022;14(19):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker
Authors: M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström
Cancers, 2022;14(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Evidence for osteocyte-mediated bone-matrix degradation associated with periprosthetic joint infection (PJI)
Authors: RT Ormsby, AR Zelmer, D Yang, NJ Gunn, Y Starczak, SP Kidd, R Nelson, LB Solomon, GJ Atkins
European cells & materials, 2021;41(0):264-280.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model
Authors: SK Kim, SD Jang, H Kim, S Chung, JK Park, HJ Kuh
Cancers (Basel), 2020;12(5):.
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF -
Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
Bone Rep, 2020;13(0):100715.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection -
Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
Authors: Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
Nat Commun, 2014;5(0):4255.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Toll-like receptor 9 ligands enhance mesenchymal stem cell invasion and expression of matrix metalloprotease-13.
Authors: Nurmenniemi S, Kuvaja P, Lehtonen S
Exp. Cell Res., 2010;316(16):2676-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Authors: Cavallo C, Desando G, Facchini A, Grigolo B
J Biomed Mater Res A, 2010;93(1):86-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Ecto-5'-nucleotidase (CD73) -mediated extracellular adenosine production plays a critical role in hepatic fibrosis.
Authors: Peng Z, Fernandez P, Wilder T, Yee H, Chiriboga L, Chan ES, Cronstein BN
FASEB J., 2008;22(7):2263-72.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
Int. J. Cancer, 2008;122(12):2805-10.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Basic fibroblast growth factor stimulates matrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cdelta pathways in human adult articular chondrocytes.
Authors: Im HJ, Muddasani P, Natarajan V, Schmid TM, Block JA, Davis F, van Wijnen AJ, Loeser RF
J. Biol. Chem., 2007;282(15):11110-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM
J. Cell. Physiol., 2007;210(2):417-27.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
The inhibition of subchondral bone resorption in the early phase of experimental dog osteoarthritis by licofelone is associated with a reduction in the synthesis of MMP-13 and cathepsin K.
Authors: Pelletier JP, Boileau C, Brunet J, Boily M, Lajeunesse D, Reboul P, Laufer S, Martel-Pelletier J
Bone, 2004;34(3):527-38.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-P
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1:100 dilution
Citrate buffer Ag retrieval
Overnight incubation of the Ab, RT
The antibody AF511 was used as the detection antibody with this antibody as the capture in an ELISA targeting MMP-13. The immunoassay was used to measure MMP-13 levels in human plasma samples.
