Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Bovine, Canine, Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation, Immunoaffinity Purification

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, ELISA Detection, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 87512
View all MMP-13 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452

Specificity

Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MMP‑13 Antibody

MMP-13 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC).

MMP‑13 in MDA‑MB‑231 Human Cell Line.

MMP-13 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Human MMP-13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MMP‑13 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑13 in Human Ovarian Cancer Tissue.

MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Canine MMP-13 by Western Blot

Detection of Canine MMP-13 by Western Blot

Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on IL-1 beta -induced NF-kappa B-dependent expression of pro-inflammatory, pro-apoptotic and matrix degrading gene products, primary chondrocytes were either stimulated with 10 ng/ml IL-1 beta, 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) or pre-stimulated for 12 h with 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) followed by 10 ng/ml IL-1 beta for 24. Equal amounts of total proteins were separated by SDS-PAGE and analyzed by immunoblotting using antibodies raised against COX-2, MMP-9 and MMP-13 and active caspase-3. Stimulation with IL-1 beta resulted in production of COX-2, MMP-9, MMP-13 and caspase-3 cleavage. Pre-treatment with a combination of both IGF-1 or/and PDGF-bb downregulated COX-2, MMP-9, MMP-13 and cleaved caspase-3. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0028663), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MMP-13 by Immunohistochemistry

Detection of Human MMP-13 by Immunohistochemistry

Enhanced MMP13 gene expression and production due to 3D osteogenic differentiation. (A) Multivariate analysis of the sixty-six tested genes (NanoString® kit) displayed as a volcano plot (created with nSolver® software) with the 2D or 3D condition set as covariate. The plot shows each gene’s −log10 (p-value) and log2 fold change associated to the 2D or 3D condition. Highly statistically significant genes fall at the top of the plot above the indicated p-value lines, and highly differentially expressed genes fall to either side (right = upregulated, left = downregulated). The only significantly (p-value < 0.01) affected gene that was upregulated was MMP13. (B) Relative gene expression of MMP13 is shown in the four various conditions: 2D undifferentiated, 2D differentiated, 3D undifferentiated, 3D differentiated, after 5 weeks of culture. The significance levels for relative gene expression difference are indicated as ** (p-value < 0.01), *** (p-value < 0.001). (C) Immunohistochemistry of 5-week undifferentiated vs. differentiated (osteogenic) 3D Col I gels against MMP13 and nuclear DAPI. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34948393), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MMP-13 by Immunohistochemistry

Detection of MMP-13 by Immunohistochemistry

Immunohistochemistry of metalloproteinase in meniscal samples. (a) number of patients with different expression of MMP-1 and -13; (b) one representative patient for MMP-1 (grade 3) and MMP-13 (grade 1), (magnification 10×, inset 40×, respectively). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/23/7/3903), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP‑13 Antibody

Application
Recommended Usage

Immunoaffinity Purification

Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human MMP-13 (Catalog # 511-MM) from conditioned media.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑13 (Catalog # 511‑MM), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑13 Western Blot Standard (Catalog # WBC020)

Reviewed Applications

Read 6 reviews rated 4.7 using MAB511 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-13

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Entrez Gene IDs

4322 (Human)

Gene Symbol

MMP13

UniProt

P45452

Additional MMP-13 Products

Product Documents for Human MMP‑13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP‑13 Antibody

For research use only

Citations for Human MMP‑13 Antibody

Customer Reviews for Human MMP‑13 Antibody (6)

4.7 out of 5
6 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
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2 Stars
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1 Stars
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Showing  1 - 5 of 6 reviews Showing All
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  • Human MMP-13 Antibody
    Name: Mozhdeh Sojoodi
    Application: Immunocytochemistry
    Sample Tested: Bone Marrrow Derived Macrophages
    Species: Mouse
    Verified Customer | Posted 02/07/2022
    Macrophages in vitro produce MMP13
    1:200 Dilution Overnight incubation at RT
    Human MMP‑13 Antibody MAB511
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Human MMP-13 Antibody
    Name: Mozhdeh Sojoodi
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 02/07/2022
    1:100 dilution Citrate buffer Ag retrieval Overnight incubation of the Ab, RT
    Human MMP‑13 Antibody MAB511
  • Human MMP-13 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate cancer tissue
    Species: Human
    Verified Customer | Posted 12/06/2021
    Human MMP‑13 Antibody MAB511
  • Human MMP-13 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Joint tissue
    Species: Human
    Verified Customer | Posted 10/14/2021
    Human MMP‑13 Antibody MAB511
  • Human MMP-13 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 07/13/2018
  • Human MMP-13 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/10/2017
    The antibody AF511 was used as the detection antibody with this antibody as the capture in an ELISA targeting MMP-13. The immunoassay was used to measure MMP-13 levels in human plasma samples.
    Human MMP‑13 Antibody MAB511

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Protocols

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