Human MMP-9 Antibody
Human MMP-9 Antibody Summary
-12 and -13 is observed.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
MMP‑9 in MMP‑9 transfected NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
MMP‑9 in Human Liver Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human MMP-9 Polyclonal Antibody (Catalog # AB911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Product Datasheets
Citations for Human MMP-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
C3d Elicits Neutrophil Degranulation and Decreases Endothelial Cell Migration, with Implications for Patients with Alpha-1 Antitrypsin Deficiency
Authors: LT Fee, D Gogoi, ME O'Brien, E McHugh, M Casey, C Gough, M Murphy, AM Hopkins, TP Carroll, NG McElvaney, EP Reeves
Biomedicines, 2021;9(12):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
J Immunol, 2015;195(5):2429-41.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
J. Immunol., 2006;177(7):4861-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
Anal. Biochem., 2005;338(2):294-8.
Species: Human
Sample Types: Plasma
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human MMP-9 Antibody
There are currently no reviews for this product. Be the first to review Human MMP-9 Antibody and earn rewards!
Have you used Human MMP-9 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
