Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Capture, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMR/CD206 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMR/CD206
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897

Specificity

Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MMR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMR/CD206 Antibody

Detection of Human MMR/CD206 antibody by Western Blot.

Detection of Human MMR/CD206 by Western Blot.

Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MMR/CD206 in Human Liver.

MMR/CD206 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human MMR/CD206 by Simple WesternTM.

Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.2 mg/mL. A specific band was detected for MMR/CD206 at approximately 201 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2534). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human MMR/CD206/Mannose Receptor by Immunohistochemistry-Paraffin

Detection of Human MMR/CD206/Mannose Receptor by Immunohistochemistry-Paraffin

Osteopontin is overexpressed in human MPM patients. (A) Real Time PCR for the Spp1 gene (osteopontin, OPN) in surgical human MPM samples. Comparison between tumor and undiseased adjacent tissues. Data are shown as mean ± SEM (Unpaired t test with Welch’s correction). (B) ELISA quantification of hOPN on plasma samples from 99 MPM patients and 101 healthy subjects. (ROUT, identify outlier and Unpaired t test with Welch’s correction). (C) Kaplan-Meier curves of overall survival according to OPN levels categorized based on CART analysis cut-off (n=61 MPM patients). (D–F) Representative images of immunohistochemistry in MPM tumor tissues stained for OPN or CD206 (40x, insert 100x) and semi-quantitative analysis in 28 cases (0=negative, 1 = 1-25% positivity, 2 = 26-50% positivity, 3= >50% positivity). Data are shown as mean ± SEM. (Unpaired t test with Welch’s correction). (G, H) ELISA quantification of hOPN on plasma samples from 18 MPM patients treated with immunotherapy. Blood was collected at baseline and after revalutation at 4-6 months. Patients with progressive disease (PD): 6 patients; stable disease (SD): 6 patients; partial response (PR): 6 patients. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37398648), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MMR/CD206 by Immunohistochemistry

Detection of MMR/CD206 by Immunohistochemistry

Cisplatin does not affect TAM viability and M2 phenotype. (A) IHC analysis of CD68, stabilin-1 and CD206 expression in human breast cancer tissue treated and not treated with cisplatin-based therapy. (B) Quantitative analysis of CD68, stabilin-1 and CD206 expression. Tumor tissue was obtained after surgery for all patients. (C) Schematic description of ex vivo TAM generation which were stimulated with MCF-7 supernatants and treated by cisplatin. (D) Immunofluorescent staining/confocal microscopy of CD206 and stabilin-1 expression in CD68+ ex vivo TAMs. Scale bars are equal to 11µM. (E) Viability assay for ex vivo TAMs treated with cisplatin. Viability was estimated on day 6 before cisplatin treatment and day 9 after cisplatin-treatment (n=6 independent experiments). Data information: In (B), the level of protein expression is presented as Me (Q1;Q3). In (E), OD means defined in the percentage of NS control are presented as mean ± SD. The Manna-Whitney test was applied to compare two independent groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36960065), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MMR/CD206 by Immunohistochemistry

Detection of MMR/CD206 by Immunohistochemistry

Cisplatin does not affect TAM viability and M2 phenotype. (A) IHC analysis of CD68, stabilin-1 and CD206 expression in human breast cancer tissue treated and not treated with cisplatin-based therapy. (B) Quantitative analysis of CD68, stabilin-1 and CD206 expression. Tumor tissue was obtained after surgery for all patients. (C) Schematic description of ex vivo TAM generation which were stimulated with MCF-7 supernatants and treated by cisplatin. (D) Immunofluorescent staining/confocal microscopy of CD206 and stabilin-1 expression in CD68+ ex vivo TAMs. Scale bars are equal to 11µM. (E) Viability assay for ex vivo TAMs treated with cisplatin. Viability was estimated on day 6 before cisplatin treatment and day 9 after cisplatin-treatment (n=6 independent experiments). Data information: In (B), the level of protein expression is presented as Me (Q1;Q3). In (E), OD means defined in the percentage of NS control are presented as mean ± SD. The Manna-Whitney test was applied to compare two independent groups. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36960065), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMR/CD206 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

10 µg/mL
Sample: Human immature dendritic cells

Western Blot

1 µg/mL
Sample: Human immature dendritic cells

Reviewed Applications

Read 4 reviews rated 3.8 using AF2534 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMR/CD206

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Gene Symbol

MRC1

UniProt

P22897

Additional MMR/CD206 Products

Product Documents for Human MMR/CD206 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMR/CD206 Antibody

For research use only

Citations for Human MMR/CD206 Antibody

Customer Reviews for Human MMR/CD206 Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human MMR/CD206 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 09/23/2019
    I used this antibody for developing a sandwich ELISA in combination with mAb (cat.MAB25341) and protein (cat.2534-MR). This combination gives good standard curve but unfortunately did not detect any MMR in our samples.
    Human MMR/CD206 Antibody AF2534
  • Human MMR/CD206 Antibody
    Name: Anonymous
    Application: Immunofluorescence - paraffin
    Sample Tested: Human spleen, FFPE
    Species: Human
    Verified Customer | Posted 04/12/2019
    FFPE human spleen, CD206, 1:100
    pH9 antigen retrieval
    Human MMR/CD206 Antibody AF2534
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22516068
    Species: Human
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22500753
    Species: Human
    Verified Customer | Posted 01/07/2015

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