Human/Mouse ADAM9 Ectodomain Antibody
Human/Mouse ADAM9 Ectodomain Antibody Summary
Ala206-Asp697
Accession # Q61072
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse ADAM9 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and WI-38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human ADAM9 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 78 and 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM9
ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane‑anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75-TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin‑binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin B-chain and fibronectin (2, 4). Besides its catalytic activity, ADAM9 functions as an adhesion molelcule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).
- Moss, M.L. et al. (2001) Drug Discov. Today 6:417.
- Roghani, M. et al. (1999) J. Biol. Chem. 274:3531.
- Izumi, Y. et al. (1998) EMBO J. 17:7260.
- Schwettmann, L. and H. Tschesche (2001) Protein. Expr. Purif. 21:65.
- Nath, D. et al. (2000) J. Cell Sci. 113:2319.
- Zhou, M. et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
- Howard, L. et al. (1999) J. Biol. Chem. 274:31693.
- Weskamp, G. et al. (1996) J. Cell Biol. 132:717.
Product Datasheets
Citations for Human/Mouse ADAM9 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Identification of theranostic factors for patients developing metastasis after surgery for early-stage lung adenocarcinoma
Authors: WC Cheng, CY Chang, CC Lo, CY Hsieh, TT Kuo, GC Tseng, SC Wong, SF Chiang, KC Huang, LC Lai, TP Lu, KSC Chao, YP Sher
Theranostics, 2021;11(8):3661-3675.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A Disintegrin and Metalloproteinase 9 Domain (ADAM9) Is a Major Susceptibility Factor in the Early Stages of Encephalomyocarditis Virus Infection
Authors: LE Bazzone, M King, CR MacKay, PP Kyawe, P Meraner, D Lindstrom, J Rojas-Quin, CA Owen, JP Wang, AL Brass, EA Kurt-Jones, RW Finberg
MBio, 2019;10(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sorting nexin 9 (SNX9) regulates levels of the transmembrane disintegrin and metalloproteinase (ADAM)-9 at the cell surface
Authors: KJ Mygind, T Störiko, ML Freiberg, J Samsøe-Pet, J Schwarz, OM Andersen, M Kveiborg
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
Oncogene, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT
Authors: CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher
Sci Rep, 2017;7(1):15108.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The association between laminin and microglial morphology in vitro
Authors: Wing Yip Tam
Sci Rep, 2016;6(0):28580.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein.
Authors: Delarasse C, Auger R, Gonnord P, Fontaine B, Kanellopoulos JM
J. Biol. Chem., 2011;286(4):2596-606.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Accelerated wound repair in ADAM-9 knockout animals.
Authors: Mauch C, Zamek J, Abety AN, Grimberg G
J. Invest. Dermatol., 2010;130(8):2120-30.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
J. Cell Biol., 2006;175(1):179-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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