Human/Mouse ADAM9 Ectodomain Antibody

(5 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse ADAM9 Ectodomain in direct ELISAs and Western blots. In Western blots no cross-reactivity with the Ectodomain of recombinant mouse ADAM10 and rhADAM8, 15, and 17 (TACE) is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse ADAM9
    Ala206-Asp697
    Accession # Q61072
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse testis
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse ADAM9 (Catalog # 949-AD), see our available Western blot detection antibodies
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse ADAM9 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF949) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM9

ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane‑anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75-TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin‑binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin B-chain and fibronectin (2, 4). Besides its catalytic activity, ADAM9 functions as an adhesion molelcule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

  • References:
    1. Moss, M.L. et al. (2001) Drug Discov. Today 6:417.
    2. Roghani, M. et al. (1999) J. Biol. Chem. 274:3531.
    3. Izumi, Y. et al. (1998) EMBO J. 17:7260.
    4. Schwettmann, L. and H. Tschesche (2001) Protein. Expr. Purif. 21:65. 
    5. Nath, D. et al. (2000) J. Cell Sci. 113:2319.
    6. Zhou, M. et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
    7. Howard, L. et al. (1999) J. Biol. Chem. 274:31693.
    8. Weskamp, G. et al. (1996) J. Cell Biol. 132:717.
  • Long Name:
    A Disintegrin and Metalloprotease-like Domain 9
  • Entrez Gene IDs:
    8754 (Human); 11502 (Mouse)
  • Alternate Names:
    a disintegrin and metalloproteinase domain 9 (meltrin gamma); ADAM 9; ADAM metallopeptidase domain 9 (meltrin gamma); ADAM metallopeptidase domain 9; ADAM9; Cellular disintegrin-related protein; cone rod dystrophy 9; CORD9; disintegrin and metalloproteinase domain-containing protein 9; EC 3.4.24; EC 3.4.24.-; MCMP; MCMPMDC9KIAA0021Mltng; MDC9; Meltrin gamma; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; MLTNG; Myeloma cell metalloproteinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
    Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. The association between laminin and microglial morphology in vitro
    Authors: Wing Yip Tam
    Sci Rep, 2016;6(0):28580.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  3. The purinergic receptor P2X7 triggers alpha-secretase-dependent processing of the amyloid precursor protein.
    Authors: Delarasse C, Auger R, Gonnord P, Fontaine B, Kanellopoulos JM
    J. Biol. Chem., 2011;286(4):2596-606.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Accelerated wound repair in ADAM-9 knockout animals.
    Authors: Mauch C, Zamek J, Abety AN, Grimberg G
    J. Invest. Dermatol., 2010;130(8):2120-30.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006;175(1):179-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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