Amphiphysin/AMPH Antibody (694121)

R&D Systems | Catalog # MAB65271

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 694121
View all Amphiphysin/AMPH Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Amphiphysin/AMPH
Asn204-Gly300
Accession # P49418

Specificity

Detects human Amphiphysin/AMPH in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Amphiphysin/AMPH Antibody (694121)

Detection of Human and Mouse Amphiphysin/ AMPH antibody by Western Blot.

Detection of Human and Mouse Amphiphysin/ AMPH by Western Blot.

Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Amphiphysin/AMPH Monoclonal Antibody (Catalog # MAB65271) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Amphiphysin/AMPH at approximately 128 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Amphiphysin/AMPH Antibody (694121)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human brain tissue and mouse brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Amphiphysin/AMPH

AMPH (Amphiphysin; also AMPH1) is a member of the Amphiphysin family, BAR superfamily of proteins. Although its calculated MW is only 78 kDa, it runs anomalously in SDS-PAGE at 125-128 kDa. It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle. It may form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation. Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization. Human AMPH is 695 amino acids (aa) in length. It contains an N-terminal BAR domain (aa 24-240) that binds lipids, a central region that binds AP2 (aa 322-340) and clathrin (aa 347-386), and a C-terminal SH3 domain (aa 622-695) that binds proline-rich ligands. There is one known 108 kDa splice variant that shows a deletion of aa 425-466. Over aa 204-300, human AMPH shares 98% aa identity with mouse AMPH.

Alternate Names

AMPH, AMPH1

Entrez Gene IDs

273 (Human); 218038 (Mouse); 60668 (Rat)

Gene Symbol

AMPH

UniProt

P49418 (Human)

Additional Amphiphysin/AMPH Products

Product Documents for Amphiphysin/AMPH Antibody (694121)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Amphiphysin/AMPH Antibody (694121)

For research use only

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