Human/Mouse Angiopoietin-1 Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Angiopoietin-2 and rhAngiopoietin-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
    Ser20-Phe498
    Accession # Q5HYA0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Angiopoietin‑1 in Human Prostate Cancer Tissue. Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Angiopoietin‑1 in Human Prostate. Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Angiopoietin‑1 in Mouse Embryo. Angiopoietin‑1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

  • References:
    1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
    2. Davis, S. et al. (1996) Cell 87:1161.
    3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
    4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
    5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
  • Entrez Gene IDs:
    284 (Human); 11600 (Mouse)
  • Alternate Names:
    AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers.
    Authors: Baruah P, Lee M, Wilson P, Odutoye T, Williamson P, Hyde N, Kaski J, Dumitriu I
    Br J Cancer, 2015;113(4):653-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
    Authors: Brunckhorst MK, Wang H, Lu R, Yu Q
    Cancer Res., 2010;70(18):7283-93.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
    Authors: Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
    FASEB J., 2008;22(8):3010-23.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
    Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
    Clin. Immunol., 2007;124(2):158-64.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  5. Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
    Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(4):L588-95.
    Species: Primate - Baboon
    Sample Type: Tissue Homogenates
    Application: WB
  6. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
    Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 application: Immunohistochemistry.

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Summary

ApplicationImmunohistochemistry
Sample TestedAdult lung
SpeciesMouse

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