Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 686106
View all Annexin A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Annexin A1
Met1-Asn346
Accession # P04083

Specificity

Detects human Annexin A1 in direct ELISAs. Detects human and mouse Annexin A1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Annexin A2, A3, A4, A6, A7, A8, A9, A10, A11, or A13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse Annexin A1 Antibody

Annexin A1 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

Annexin A1 in NIH-3T3 Mouse Cell Line.

Annexin A1 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line (left panel, positive stain) and NIH-3T3 Knockouts (right panel, negative stain) using Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB3770) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei, cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Annexin A1 antibody in Human Breast by Immunohistochemistry (IHC-P).

Annexin A1 in Human Breast.

Annexin A1 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB3770) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to myoepithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse Annexin A1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Knockout Validated

Annexin A1 is specifically detected in NIH- 3T3 mouse embryonic fibroblast cell lineparental cell line but is not detectable in Annexin A1 knockout NIH- 3T3 cell line.

Reviewed Applications

Read 1 review rated 5 using MAB3770 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin A1

The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A1 (ANXA1), also known as annexin I, lipocortin I, and calpactin II, is an ~ 40 kDa protein with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, Annexin A1 may have anti-inflammatory activity. Human Annexin A1 shares 88 and 89% identity with mouse and rat Annexin A1, respectively.

Alternate Names

ANX1, ANXA1, LPC1

Entrez Gene IDs

301 (Human); 16952 (Mouse); 25380 (Rat)

Gene Symbol

ANXA1

UniProt

P04083

Additional Annexin A1 Products

Product Documents for Human/Mouse Annexin A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Annexin A1 Antibody

For research use only

Citations for Human/Mouse Annexin A1 Antibody

Customer Reviews for Human/Mouse Annexin A1 Antibody (1)

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  • Human/Mouse Annexin A1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lymph node tissue
    Species: Human
    Verified Customer | Posted 07/25/2023
    IHC-P: pH 9 antigen retrieval, primary Ab x1000, HRP-DAB detection system
    Human/Mouse Annexin A1 Antibody MAB3770

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Protocols

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