Human/Mouse BACE-1 Ectodomain Antibody
Human/Mouse BACE-1 Ectodomain Antibody Summary
Thr22-Tyr460
Accession # P56817
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BACE‑1 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE-1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-1
BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6‑8).
- Vassar, R. et al. (1999) Science 286:735.
- Yan, R. et al. (1999) Nature 402:533.
- Cai, H. et al. (2001) Nature Neurosci. 4:233.
- Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
- Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
- Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
- Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
- Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.
Product Datasheets
Citations for Human/Mouse BACE-1 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Abeta Peptide Levels in SweAPP N2a Cells
Authors: MI Khan, JH Shin, MY Kim, TS Shin, JD Kim
Molecules, 2020;25(10):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
Sci Adv, 2020;6(9):eaax3223.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology
Authors: A Mendsaikha, I Tooyama, JP Bellier, GE Serrano, LI Sue, LF Lue, TG Beach, DG Walker
Acta Neuropathol Commun, 2019;7(1):215.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Deficiency of Neuronal p38alpha MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
Authors: Schnoder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y
J Biol Chem, 2015;291(5):2067-79.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
High activities of BACE1 in brains with mild cognitive impairment.
Authors: Cheng X, He P, Lee T, Yao H, Li R, Shen Y
Am J Pathol, 2014;184(1):141-7.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology.
Authors: Wang Y, Thinakaran G, Kar S
PLoS ONE, 2014;9(5):e98057.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
Authors: Ahmed RR, Holler CJ, Webb RL
J. Neurochem., 2010;112(4):1045-53.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development
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