Human/Mouse BACE-1 Ectodomain Antibody MAB931-100: R&D Systems

Human/Mouse BACE-1 Ectodomain Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse BACE-1 in direct ELISAs and human BACE-1 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BACE-2, recombinant mouse (rm) BACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 137612
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BACE-1
    Thr22-Tyr460
    Accession # P56817
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human BACE‑1 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE‑1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE‑1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-1
BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma  secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6‑8).
  • References:
    1. Vassar, R. et al. (1999) Science 286:735.
    2. Yan, R. et al. (1999) Nature 402:533.
    3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
    4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
    5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
    6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
    7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
    8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.
  • Long Name:
    beta-site Ayloid Precursor Protein Cleaving Enzyme 1
  • Entrez Gene IDs:
    23621 (Human); 23821 (Mouse)
  • Alternate Names:
    APP beta-secretase; ASP2; Aspartyl protease 2; BACE1; BACE-1; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; beta-site APP-cleaving enzyme; EC 3.4.23; EC 3.4.23.46; FLJ90568; HSPC104; KIAA1149; memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Deficiency of Neuronal p38alpha MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
    Authors: Schnoder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y
    J Biol Chem, 2015;291(5):2067-79.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. High activities of BACE1 in brains with mild cognitive impairment.
    Authors: Cheng X, He P, Lee T, Yao H, Li R, Shen Y
    Am J Pathol, 2014;184(1):141-7.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  3. Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology.
    Authors: Wang Y, Thinakaran G, Kar S
    PLoS ONE, 2014;9(5):e98057.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
    Authors: Ahmed RR, Holler CJ, Webb RL
    J. Neurochem., 2010;112(4):1045-53.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
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