Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Bad Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Bad synthetic peptide
MFQIPEFEPSEQEDSSSAERGC
Accession # Q92934

Specificity

Detects human and mouse Bad in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Bad aa 1‑21 Antibody

Detection of Human/Mouse Bad antibody by Western Blot.

Detection of Human/Mouse Bad by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bad at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Bad antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

Bad in Jurkat Human Cell Line.

Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse Bad aa 1‑21 Antibody

Application
Recommended Usage

Immunocytochemistry

0.3-15 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L‑929 mouse fibroblast cell line

Reviewed Applications

Read 2 reviews rated 4 using AF819 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bad

Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Gene Symbol

BAD

UniProt

Q92934 (Human)

Additional Bad Products

Product Documents for Human/Mouse Bad aa 1‑21 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Bad aa 1‑21 Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human/Mouse Bad aa 1‑21 Antibody

Customer Reviews for Human/Mouse Bad aa 1‑21 Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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2 Stars
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1 Stars
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Customer Images

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  • Human/Mouse Bad aa 1‑21 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human/Mouse Bad aa 1‑21 Antibody AF819
  • Human/Mouse Bad aa 1‑21 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung tissue and Lung single-cell suspension
    Species: Mouse
    Verified Customer | Posted 06/05/2019
    Human/Mouse Bad aa 1‑21 Antibody AF819

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