Human/Mouse Bax Antibody

  (4 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (4)
FAQs
Supplemental Products
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  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Bax.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    KLH-coupled human Bax synthetic peptide
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.3 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    1-15 µg/mL
    See below
  • Knockout Validated
    Bax is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bax knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse Bax by Western Blot. Western blot shows lysates of CHP‑100 human neuroblastoma cell line, THP‑1 human acute monocytic leukemia cell line, L‑929 mouse fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
Bax in A549 Human Cell Line. Bax was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Bax in Human Breast. Bax was detected in immersion fixed paraffin-embedded sections of human breast using 15 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained (red) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Bax in Human Liver. Bax was detected in immersion fixed paraffin-embedded sections of human liver using 10 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP‑DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Knockout Validated
Western Blot Shows Human Bax Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bax
Bax belongs to the Bcl2 protein family. It forms homodimers and heterodimers with Bcl2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.
  • Long Name:
    Bcl Associated X Protein
  • Entrez Gene IDs:
    581 (Human); 12028 (Mouse); 24887 (Rat)
  • Alternate Names:
    apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Bcl-xL Is Essential for the Survival and Function of Differentiated Neurons in the Cortex That Control Complex Behaviors
    J Neurosci, 2016;36(20):5448-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006;56(2):243-52.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes.
    Authors: Xi H, Kersh GJ
    J. Immunol., 2004;173(1):340-8.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  4. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
    Authors: Jayanthi S, Deng X, Bordelon M, McCoy MT, Cadet JL
    FASEB J., 2001;15(10):1745-52.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB

FAQs

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Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Rabbit IgG Control

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Normal Rabbit IgG Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

WB HAF008 35  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit IgG Fluorescein-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse Bax Antibody AF820 Array AF820
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Excellent
 WB Human Anonymous 01/11/2018
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Western Blot Human/Mouse Bax Antibody AF820
Western Blot: Human/Mouse Bax Antibody [AF820].

Summary

Application Western Blot
Sample Tested MDA-MB-231 human breast cancer cell line
Species Human

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