Human/Mouse Bax Antibody

R&D Systems | Catalog # AF820

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Bax Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Bax synthetic peptide

Specificity

Detects human and mouse Bax.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Bax Antibody

Detection of Human/Mouse Bax antibody by Western Blot.

Detection of Human/Mouse Bax by Western Blot.

Western blot shows lysates of CHP-100 human neuroblastoma cell line, THP-1 human acute monocytic leukemia cell line, L-929 mouse fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Bax antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

Bax in A549 Human Cell Line.

Bax was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Bax antibody in Human Breast by Immunohistochemistry (IHC-P).

Bax in Human Breast.

Bax was detected in immersion fixed paraffin-embedded sections of human breast using 15 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained (red) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Bax antibody in Human Liver by Immunohistochemistry (IHC-P).

Bax in Human Liver.

Bax was detected in immersion fixed paraffin-embedded sections of human liver using 10 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human Bax Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Bax Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Bax antibody by Simple WesternTM.

Detection of Human Bax by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Bax at approximately 26 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Bax by Western Blot

Detection of Bax by Western Blot

LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of (a) NLRP3, ASC and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1 beta and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Bax by Western Blot

Detection of Bax by Western Blot

Glycyrrhizic acid induces the apoptosis of colorectal cancer cells by inhibiting SIRT3 in vitro. (a,b) 0 or 20 μmol/L glycyrrhizin stimulated colorectal cancer cells for 24 hours, flow cytometry was used to detect the apoptosis of colorectal cancer cells (a) and statistical comparison (b). (c) Western blot analysis of Bax and Bcl2 protein expression in colorectal cancer cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. (d) Elisa kit was used to determine the activity of caspase 3 in colorectal cancer cells after stimulating with 0 or 20 μmol/L glycyrrhizic acid for 24 hours. Each test is repeated at least 3 times independently. *** P < 0.001 vs WT group, and ### P < 0.001 vs 20 μM GA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34747319), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Bax Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and immersion fixed paraffin-embedded sections of human breast

Knockout Validated

Bax is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bax knockout HeLa cell line.

Simple Western

50 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

0.3 µg/mL
Sample: CHP-100 human neuroblastoma cell line, THP-1 human acute monocytic leukemia cell line, L-929 mouse fibroblast cell line, and DA3 mouse myeloma cell line

Reviewed Applications

Read 4 reviews rated 4.3 using AF820 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bax

Bax belongs to the Bcl2 protein family. It forms homodimers and heterodimers with Bcl2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Gene Symbol

BAX

Additional Bax Products

Product Documents for Human/Mouse Bax Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Bax Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human/Mouse Bax Antibody

Customer Reviews for Human/Mouse Bax Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse Bax Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 07/30/2019
    Human/Mouse Bax Antibody AF820
  • Human/Mouse Bax Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Brain (cortex) tissue
    Species: Rat
    Verified Customer | Posted 01/23/2018
    Missing band in C1:7 lane.
    Human/Mouse Bax Antibody AF820
  • Human/Mouse Bax Antibody
    Name: Jothi Jayavel
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/11/2018
    Human/Mouse Bax Antibody AF820
  • Name: Anonymous
    Application: Simple Western
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 09/14/2015
    Bax AF820
    Human/Mouse Bax Antibody AF820

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Protocols

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