Human/Mouse Bax Antibody

Catalog # Availability Size / Price Qty
AF820
Detection of Human/Mouse Bax by Western Blot.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse Bax Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bax.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled human Bax synthetic peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
1-15 µg/mL
See below
Knockout Validated
Bax is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bax knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse Bax antibody by Western Blot. View Larger

Detection of Human/Mouse Bax by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line, THP-1 human acute monocytic leukemia cell line, L-929 mouse fibroblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry Bax antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

Bax in A549 Human Cell Line. Bax was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Bax antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

Bax in Human Breast. Bax was detected in immersion fixed paraffin-embedded sections of human breast using 15 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained (red) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Bax antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Bax in Human Liver. Bax was detected in immersion fixed paraffin-embedded sections of human liver using 10 µg/mL Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human Bax Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Bax Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bax at approximately 20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Bax antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Bax by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bax knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Bax at approximately 26 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human/Mouse Bax Antigen Affinity-purified Polyclonal Antibody (Catalog # AF820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bax

Bax belongs to the Bcl2 protein family. It forms homodimers and heterodimers with Bcl2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.

Long Name
Bcl Associated X Protein
Entrez Gene IDs
581 (Human); 12028 (Mouse); 24887 (Rat)
Alternate Names
apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4

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Citations for Human/Mouse Bax Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Human Umbilical Cord Mesenchymal Stem Cells Attenuate Severe Burn-Induced Multiple Organ Injury via Potentiating IGF-1 and BCL-2/BAX Pathway
    Authors: H Wang, T Ba, Q Wang, L Yang, C Li, X Hao, Y Yin, L Liu
    Stem Cells International, 2022-12-23;2022(0):5474289.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
    Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
    International immunopharmacology, 2021-12-29;103(0):108494.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
    Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
    Mol Med Rep, 2019-06-06;20(2):879-886.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Bcl-xL Is Essential for the Survival and Function of Differentiated Neurons in the Cortex That Control Complex Behaviors
    J Neurosci, 2016-05-18;36(20):5448-61.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes.
    Authors: Xi H, Kersh GJ
    J. Immunol., 2004-07-01;173(1):340-8.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Methamphetamine causes differential regulation of pro-death and anti-death Bcl-2 genes in the mouse neocortex.
    Authors: Jayanthi S, åÏctÐs, Deng X, Bordelon M, McCoy MT, Cadet JL
    2001-08-01;15(10):1745-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Human/Mouse Bax Antibody
By Anonymous on 07/30/2019
Application: ELISA Sample Tested: Cancer cell lysates Species: Human

Human/Mouse Bax Antibody
By Jothi Jayavel on 01/11/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human