Human/Mouse c-Rel Antibody

Catalog # Availability Size / Price Qty
AF2699
AF2699-SP

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Detection of Human/Mouse c-Rel by Western Blot.
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Product Details
Citations (2)
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Reviews (1)

Human/Mouse c-Rel Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse c-Rel in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # NP_033070
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse c-Rel antibody by Western Blot. View Larger

Detection of Human/Mouse c-Rel by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of c-Rel-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of c‑Rel-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL and 200 ng/mL PMA and calcium ionomycin for overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. c-Rel/DNA complexes were immuno-precipitated using 5 µg Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.

Immunocytochemistry c-Rel antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

c-Rel in THP-1 Human Cell Line. c-Rel was detected in immersion fixed THP-1 human acute monocytic leukemia cell line treated with LPS using Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei upon stimulation with LPS. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection of Mouse c-Rel antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse c‑Rel by Simple WesternTM. Simple Western lane view shows lysates of CH-1 mouse B cell lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Rel at approximately 71 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Rel

c-Rel/Rel, the cellular counterpart of the v-Rel oncogene of the avian reticuloendotheliosis retrovirus, is a 69 kDa class II member of the Rel/NK-kappa B family of transcription factors. All Rel family members contain a RHD (Rel homology domain) that is involved in dimerization, DNA and I kappa B binding, and nuclear localization. Class II members contain an additional C-terminal transcriptional activation segment. Rel both homodimerizes and heterodimerizes with multiple family members. Following dimerization, Rel complexes initiate transcription by acting on decameric DNA motifs termed kappa B binding sites. The important role of c-Rel in B-cell development, growth, and survival is well documented. c-Rel is also involved in responses to auto-antigens, allo-antigens, allergens and pathogens and may contribute to the development of certain human lymphoid cell cancers.

Long Name
Cellular Repressor of E1A-stimulated Genes
Entrez Gene IDs
5966 (Human); 19696 (Mouse)
Alternate Names
C-Rel proto-oncogene protein; cRel; c-Rel; I-Rel; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; REL; v-rel avian reticuloendotheliosis viral oncogene homolog; v-rel reticuloendotheliosis viral oncogene homolog (avian)

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Citations for Human/Mouse c-Rel Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. c-Rel gain in B cells drives germinal center reactions and autoantibody production
    Authors: M Kober-Hass, H Oh-Strau beta, D Kumar, V Soberón, C Diehl, M Lech, T Engleitner, E Katab, V Fernandez, G Piontek, H Li, B Menze, C Ziegenhain, W Enard, R Rad, JP Böttcher, HJ Anders, M Rudelius, M Schmidt-Su
    J. Clin. Invest., 2020-06-01;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice.
    Authors: Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
    J. Immunol., 2012-06-18;189(2):1053-61.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse c-Rel Antibody

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Human/Mouse c-Rel Antibody
By Anonymous on 06/18/2018
Application: IHC Sample Tested: Paraffined tumor tissue Species: Human

Citrate ph:6 based antigen retrieval
1:50 primary AB dilution, o/n 4C incubation, dilution factor can be increased
ABComplex-DAB developing