Human/Mouse c-Rel Antibody Summary
Accession # NP_033070
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse c-Rel by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of c‑Rel-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL and 200 ng/mL PMA and calcium ionomycin for overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. c-Rel/DNA complexes were immuno-precipitated using 5 µg Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.
c-Rel in THP-1 Human Cell Line. c-Rel was detected in immersion fixed THP-1 human acute monocytic leukemia cell line treated with LPS using Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei upon stimulation with LPS. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Mouse c‑Rel by Simple WesternTM. Simple Western lane view shows lysates of CH-1 mouse B cell lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Rel at approximately 71 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
c-Rel/Rel, the cellular counterpart of the v-Rel oncogene of the avian reticuloendotheliosis retrovirus, is a 69 kDa class II member of the Rel/NK-kappa B family of transcription factors. All Rel family members contain a RHD (Rel homology domain) that is involved in dimerization, DNA and I kappa B binding, and nuclear localization. Class II members contain an additional C-terminal transcriptional activation segment. Rel both homodimerizes and heterodimerizes with multiple family members. Following dimerization, Rel complexes initiate transcription by acting on decameric DNA motifs termed kappa B binding sites. The important role of c-Rel in B-cell development, growth, and survival is well documented. c-Rel is also involved in responses to auto-antigens, allo-antigens, allergens and pathogens and may contribute to the development of certain human lymphoid cell cancers.
Citations for Human/Mouse c-Rel Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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c-Rel gain in B cells drives germinal center reactions and autoantibody production
Authors: M Kober-Hass, H Oh-Strau beta, D Kumar, V Soberón, C Diehl, M Lech, T Engleitner, E Katab, V Fernandez, G Piontek, H Li, B Menze, C Ziegenhain, W Enard, R Rad, JP Böttcher, HJ Anders, M Rudelius, M Schmidt-Su
J. Clin. Invest., 2020;0(0):.
Sample Types: Cell Culture Lysates
Applications: Western Blot
TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice.
Authors: Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
J. Immunol., 2012;189(2):1053-61.
Sample Types: Cell Lysates
Applications: Western Blot
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