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Human/Mouse COX-1 Antibody

Catalog #: MAB37401
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB37401
MAB37401-SP

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Detection of Human and Mouse COX‑1 by Western Blot.
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Human/Mouse COX-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human COX-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) COX-4I1 and no cross-reactivity with rhCOX-1 or rhCOX-4I2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 596002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human COX-1
Asn381-Leu599
Accession # P23219
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse COX-1 antibody by Western Blot. View Larger

Detection of Human and Mouse COX‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and B16-F10 mouse melanoma cell line. PVDF Membrane was probed with 2 µg/mL of Human COX-1 Monoclonal Antibody (Catalog # MAB37401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX-1

COX-1 is an approximately 70 kDa protein that is associated with microsomal membranes. COX-1 participates in the synthesis of prostaglandins and is the enzymatic target of non-steroidal anti-inflammatory drugs. A splice variant of COX-1 is generated by internal deletion of 37 residues. Human COX-1 shares 88%‑90% amino acid sequence identity with mouse and rat COX‑1.

Long Name
Cyclooxygenase 1
Entrez Gene IDs
5742 (Human); 19224 (Mouse); 24693 (Rat)
Alternate Names
COX1; COX-1; COX1PGG/HS; COX3; Cyclooxygenase-1; EC 1.14.99.1; PCOX1; PGH synthase 1; PGHS1; PGHS-1PHS1; PHS 1; prostaglandin G/H synthase 1; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 1; PTGHS; PTGS1

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Citation for Human/Mouse COX-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expression
    Authors: C Tang, H Sun, M Kadoki, W Han, X Ye, Y Makusheva, J Deng, B Feng, D Qiu, Y Tan, X Wang, Z Guo, C Huang, S Peng, M Chen, Y Adachi, N Ohno, S Trombetta, Y Iwakura
    Nature Communications, 2023-03-17;14(1):1493.

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