Human/Mouse COX-1 Antibody

Catalog #: MAB37401 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB37401
MAB37401-SP

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Detection of Human and Mouse COX‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse COX-1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human COX-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) COX-4I1 and no cross-reactivity with rhCOX-1 or rhCOX-4I2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 596002

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human COX-1
Asn381-Leu599
Accession # P23219

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse COX‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and B16-F10 mouse melanoma cell line. PVDF Membrane was probed with 2 µg/mL of Human COX-1 Monoclonal Antibody (Catalog # MAB37401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX-1

COX-1 is an approximately 70 kDa protein that is associated with microsomal membranes. COX-1 participates in the synthesis of prostaglandins and is the enzymatic target of non-steroidal anti-inflammatory drugs. A splice variant of COX-1 is generated by internal deletion of 37 residues. Human COX-1 shares 88%‑90% amino acid sequence identity with mouse and rat COX‑1.

Long Name

Cyclooxygenase 1

Entrez Gene IDs

5742 (Human); 19224 (Mouse); 24693 (Rat)

Alternate Names

COX1; COX-1; COX1PGG/HS; COX3; cyclooxygenase-1; EC 1.14.99.1; PCOX1; PGH synthase 1; PGHS1; PGHS-1PHS1; PHS 1; prostaglandin G/H synthase 1; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 1; PTGHS; PTGS1