COX-1 Antibody (596002)

R&D Systems | Catalog # MAB37401

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 596002
View all COX-1 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human COX-1
Asn381-Leu599
Accession # P23219

Specificity

Detects human COX-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) COX-4I1 and no cross-reactivity with rhCOX-1 or rhCOX-4I2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for COX-1 Antibody (596002)

Detection of Human and Mouse COX-1 antibody by Western Blot.

Detection of Human and Mouse COX‑1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and B16-F10 mouse melanoma cell line. PVDF Membrane was probed with 2 µg/mL of Human COX-1 Monoclonal Antibody (Catalog # MAB37401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for COX-1 Antibody (596002)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and B16‑F10 mouse melanoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: COX-1

COX-1 is an approximately 70 kDa protein that is associated with microsomal membranes. COX-1 participates in the synthesis of prostaglandins and is the enzymatic target of non-steroidal anti-inflammatory drugs. A splice variant of COX-1 is generated by internal deletion of 37 residues. Human COX-1 shares 88%‑90% amino acid sequence identity with mouse and rat COX‑1.

Long Name

Cyclooxygenase 1

Alternate Names

COX1, PTGS1

Entrez Gene IDs

5742 (Human); 19224 (Mouse); 24693 (Rat)

Gene Symbol

PTGS1

UniProt

P23219

Additional COX-1 Products

Product Documents for COX-1 Antibody (596002)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for COX-1 Antibody (596002)

For research use only

Citations for COX-1 Antibody (596002)

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