Human/Mouse Glypican 5 Antibody

Catalog # Availability Size / Price Qty
MAB2607
MAB2607-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Glypican 5 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.
Source
Monoclonal Mouse IgG2A Clone # 297716
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 5
Glu25-Thr554
Accession # P78333
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Glypican 5 (Catalog # 2607-G5)
Recombinant Mouse Glypican 5 (Catalog # 2689-G5)
Flow Cytometry
2.5 µg/106 cells
H9 human cutaneous T lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 5

Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. Glypican 5 is expressed primarily in embryonic neurons and mesenchyme.

Entrez Gene IDs
2262 (Human); 103978 (Mouse)
Alternate Names
bA93M14.1; Glypican 5; glypican proteoglycan 5; glypican-5; GPC5

Product Datasheets

Citations for Human/Mouse Glypican 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT
    Authors: M Takeuchi, K Takeuchi, T Takai, R Yamaguchi, T Furukawa, KI Akagi, JK Takeuchi
    PLoS ONE, 2021;16(2):e0226538.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Heparan sulfate proteoglycans containing a glypican 5 core and 2-O-sulfo-iduronic acid function as Sonic Hedgehog co-receptors to promote proliferation.
    Authors: Witt, Rochelle, Hecht, Marie-Ly, Pazyra-Murphy, Maria F, Cohen, Samuel M, Noti, Christia, van Kuppevelt, Toin H, Fuller, Maria, Chan, Jennifer, Hopwood, John J, Seeberger, Peter H, Segal, Rosalind
    J Biol Chem, 2013;288(36):26275-88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Role for amplification and expression of glypican-5 in rhabdomyosarcoma.
    Authors: Williamson D, Selfe J, Lu YJ, Pritchard-Jones K, Murai K, Jones P, Workman P, Shipley J
    Cancer Res., 2007;67(1):57-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Glypican 5 Antibody

Average Rating: 4 (Based on 1 Review)

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Human/Mouse Glypican 5 Antibody
By Anonymous on 03/15/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: UE6E7T-3 Species: Human