Human/Mouse HO-2/HMOX2 Antibody Summary
Accession # P30519
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse HO‑2/HMOX2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse HO‑2/HMOX2 Monoclonal Antibody (Catalog # MAB3170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO‑2/HMOX2 was detected at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, the constitutively expressed Heme Oxygenase-2(HO-2/HMOX2) and the inducible Heme Oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.
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