Human/Mouse HO-2/HMOX2 Antibody

Catalog # Availability Size / Price Qty
Human/Mouse HO-2/HMOX2 Antibody in Western Blot
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Product Details
Supplemental Products

Human/Mouse HO-2/HMOX2 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse HO‑2/HMOX2 in Western blots. Endogenous rat HO-2 is detectable but has a higher background. In Western blots, no cross-reactivity with recombinant human HO-1/HMOX1 is observed.
Monoclonal Mouse IgG2A Clone # 322913
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HO‑2/HMOX2
Accession # P30519
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse HO‑2/HMOX2 by Western Blot. View Larger

Detection of Human and Mouse HO‑2/HMOX2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse HO-2/HMOX2 Monoclonal Antibody (Catalog # MAB3170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-2/HMOX2 was detected at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HO-2/HMOX2

Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, the constitutively expressed Heme Oxygenase-2(HO-2/HMOX2) and the inducible Heme Oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.

Long Name
Heme Oxygenase 2
Entrez Gene IDs
3163 (Human); 15369 (Mouse); 79239 (Rat)
Alternate Names
heme oxygenase (decycling) 2; heme oxygenase 2; HMOX2; HO2; HO-2; HO-2EC

Product Datasheets


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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