Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 503404
View all IRF6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRF6
Ala176-Gln366
Accession # O14896

Specificity

Detects human and mouse IRF6 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse IRF6 Antibody

Detection of Human and Mouse IRF6 antibody by Western Blot.

Detection of Human and Mouse IRF6 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IRF6 Monoclonal Antibody (Catalog # MAB6114) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF6 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse IRF6 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRF6

Interferon regulatory factor 6 (IRF6), is a transcription factor that controls the proliferation and differentiation of epidermal and epithelial cells. IRF6 interacts with maspin in normal mammary epithelial cells but is down‑regulated during tumor progression. IRF6 polymorphisms cause Van der Woude and popliteal pterygium syndromes of skin, limb, and craniofacial development that are characterized by cleft lip, cleft palate, or lip pits. IRF6 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within aa 176‑366 of the C‑terminal domain, human IRF6 shares 98% aa sequence identity with mouse and rat IRF6.

Long Name

Interferon Regulatory Factor 8

Alternate Names

LPS, OFC6, PIT, PPS, VWS

Entrez Gene IDs

3664 (Human); 54139 (Mouse); 364081 (Rat)

Gene Symbol

IRF6

UniProt

O14896

Additional IRF6 Products

Product Documents for Human/Mouse IRF6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse IRF6 Antibody

For research use only

Citations for Human/Mouse IRF6 Antibody

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