Human/Mouse IRF6 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse IRF6 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human/Mouse IRF6 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse IRF6 in Western blots.
Monoclonal Mouse IgG2B Clone # 503404
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IRF6
Accession # O14896
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse IRF6 antibody by Western Blot. View Larger

Detection of Human and Mouse IRF6 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IRF6 Monoclonal Antibody (Catalog # MAB6114) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF6 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF6

Interferon regulatory factor 6 (IRF6), is a transcription factor that controls the proliferation and differentiation of epidermal and epithelial cells. IRF6 interacts with maspin in normal mammary epithelial cells but is down‑regulated during tumor progression. IRF6 polymorphisms cause Van der Woude and popliteal pterygium syndromes of skin, limb, and craniofacial development that are characterized by cleft lip, cleft palate, or lip pits. IRF6 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within aa 176‑366 of the C‑terminal domain, human IRF6 shares 98% aa sequence identity with mouse and rat IRF6.

Long Name
Interferon Regulatory Factor 8
Entrez Gene IDs
3664 (Human); 54139 (Mouse); 364081 (Rat)
Alternate Names
interferon regulatory factor 6; IRF6; IRF-6; LPS; OFC6; OFC6PPS; PIT; PPS; Van der Woude syndrome; VWS; VWS1

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Citation for Human/Mouse IRF6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Genetic factors define CPO and CLO subtypes of nonsyndromicorofacial cleft
    Authors: L Huang, Z Jia, Y Shi, Q Du, J Shi, Z Wang, Y Mou, Q Wang, B Zhang, Q Wang, S Ma, H Lin, S Duan, B Yin, Y Lin, Y Wang, D Jiang, F Hao, L Zhang, H Wang, S Jiang, H Xu, C Yang, C Li, J Li, B Shi, Z Yang
    PLoS Genet., 2019;15(10):e1008357.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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Reconstitution Buffers

Secondary Antibodies

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