Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MAGI2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MAGI2
Ser2-Arg130
Accession # Q86UL8

Specificity

Detects human MAGI2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant ARIP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse MAGI2 Antibody

Detection of Human MAGI2 antibody by Western Blot.

Detection of Human MAGI2 by Western Blot.

Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAGI2 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MAGI2 antibody in U-87 MG Human Cell Line by Immunocytochemistry (ICC).

MAGI2 in U‑87 MG Human Cell Line.

MAGI2 was detected in immersion fixed U-87 MG human glioblastoma/ astrocytoma cell line using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MAGI2 antibody in Human Brain by Immunohistochemistry (IHC-P).

MAGI2 in Human Brain.

MAGI2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons and neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse MAGI2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Western Blot

1 µg/mL
Sample: Human brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAGI2

MAGI2 (AIP-1, ACVRINP1 also known as Activin receptor-interacting protein 1 and S-SCAM in rodents);  is a 160‑180 kDa member of the MAGUK family of proteins. It is found in neuronal post-synaptic membrane complexes, and serves as a molecular scaffold for multiple proteins, including alpha -actinin, dendrin, SMAD3 and beta ‑catenin. ARIP-1 facilitates the signaling of both growth factor and neurotransmitter receptors such as ActRIIA, NMDA and beta 1-adrenergic receptors. Human ARIP-1 is 1455 amino acids (aa) in length. It contains an N-terminal PZD domain (aa 17-101), followed by a guanylate kinase-like domain (aa 109-283), two WW domains (aa 302‑381) and five subsequent PZD domains (aa 426-1229). ARIP-1 is reported to dimerize/oligomerize. There are three potential isoform variants. All utilize an alternative start site at Met164 that may be accompanied by either an Arg substitution for aa 757-771, or a 48 aa substitution for aa 1236-1455. Over aa 2-130, human and mouse ARIP-1 are identical in aa sequence.

Long Name

Membrane-associate Guanylate Kinase Inverted 2

Alternate Names

ACVRINP1, ACVRIP1, AIP1, ARIP1, SSCAM

Entrez Gene IDs

9863 (Human); 50791 (Mouse); 113970 (Rat)

Gene Symbol

MAGI2

UniProt

Q86UL8 (Human)

Additional MAGI2 Products

Product Documents for Human/Mouse MAGI2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse MAGI2 Antibody

For research use only

Citations for Human/Mouse MAGI2 Antibody

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Protocols

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