Human/Mouse MAGI2 Antibody

Catalog # Availability Size / Price Qty
AF7117
AF7117-SP
Detection of Human MAGI2 by Western Blot.
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Product Details
Citations (1)
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Human/Mouse MAGI2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human MAGI2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant ARIP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MAGI2
Ser2-Arg130
Accession # Q86UL8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MAGI2 by Western Blot. View Larger

Detection of Human MAGI2 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAGI2 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry MAGI2 in U‑87 MG Human Cell Line. View Larger

MAGI2 in U‑87 MG Human Cell Line.

MAGI2 was detected in immersion fixed U‑87 MG human glioblastoma/
astrocytoma cell line using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MAGI2 in Human Brain. View Larger

MAGI2 in Human Brain. MAGI2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons and neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAGI2

MAGI2 (AIP-1, ACVRINP1 also known as Activin receptor-interacting protein 1 and S-SCAM in rodents);  is a 160‑180 kDa member of the MAGUK family of proteins. It is found in neuronal post-synaptic membrane complexes, and serves as a molecular scaffold for multiple proteins, including alpha -actinin, dendrin, SMAD3 and beta ‑catenin. ARIP-1 facilitates the signaling of both growth factor and neurotransmitter receptors such as ActRIIA, NMDA and beta 1-adrenergic receptors. Human ARIP-1 is 1455 amino acids (aa) in length. It contains an N-terminal PZD domain (aa 17-101), followed by a guanylate kinase-like domain (aa 109-283), two WW domains (aa 302‑381) and five subsequent PZD domains (aa 426-1229). ARIP-1 is reported to dimerize/oligomerize. There are three potential isoform variants. All utilize an alternative start site at Met164 that may be accompanied by either an Arg substitution for aa 757-771, or a 48 aa substitution for aa 1236-1455. Over aa 2-130, human and mouse ARIP-1 are identical in aa sequence.

Long Name
Membrane-associate Guanylate Kinase Inverted 2
Entrez Gene IDs
9863 (Human); 50791 (Mouse); 113970 (Rat)
Alternate Names
ACVRINP1; ACVRIP1; AIP1; AIP1AIP-1; ARIP1; atrophin 1 interacting protein 1; atrophin-1 interacting protein A; Atrophin-1-interacting protein 1; Atrophin-1-interacting protein A; KIAA0705activin receptor interacting protein 1; MAGI2; MAGI-2SSCAM; membrane associated guanylate kinase, WW and PDZ domain containing 2; Membrane-associated guanylate kinase inverted 2; membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2; SSCAM

Product Datasheets

Citation for Human/Mouse MAGI2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage
    Authors: Z Zuo, JX Shen, Y Pan, J Pu, YG Li, XH Shao, WP Wang
    Cell. Physiol. Biochem., 2018;51(1):244-261.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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