Human/Mouse MAGI2 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human MAGI2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant ARIP-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MAGI2
    Ser2-Arg130
    Accession # Q86UL8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MAGI2 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAGI2 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
MAGI2 in U‑87 MG Human Cell Line.

MAGI2 was detected in immersion fixed U‑87 MG human glioblastoma/
astrocytoma cell line using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry
MAGI2 in Human Brain. MAGI2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human MAGI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7117) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons and neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAGI2

MAGI2 (AIP-1, ACVRINP1 also known as Activin receptor-interacting protein 1 and S-SCAM in rodents);  is a 160‑180 kDa member of the MAGUK family of proteins. It is found in neuronal post-synaptic membrane complexes, and serves as a molecular scaffold for multiple proteins, including alpha -actinin, dendrin, SMAD3 and beta ‑catenin. ARIP-1 facilitates the signaling of both growth factor and neurotransmitter receptors such as ActRIIA, NMDA and beta 1-adrenergic receptors. Human ARIP-1 is 1455 amino acids (aa) in length. It contains an N-terminal PZD domain (aa 17-101), followed by a guanylate kinase-like domain (aa 109-283), two WW domains (aa 302‑381) and five subsequent PZD domains (aa 426-1229). ARIP-1 is reported to dimerize/oligomerize. There are three potential isoform variants. All utilize an alternative start site at Met164 that may be accompanied by either an Arg substitution for aa 757-771, or a 48 aa substitution for aa 1236-1455. Over aa 2-130, human and mouse ARIP-1 are identical in aa sequence.

  • Long Name:
    Membrane-associate Guanylate Kinase Inverted 2
  • Entrez Gene IDs:
    9863 (Human); 50791 (Mouse); 113970 (Rat)
  • Alternate Names:
    ACVRINP1; ACVRIP1; AIP1; AIP1AIP-1; ARIP1; atrophin 1 interacting protein 1; atrophin-1 interacting protein A; Atrophin-1-interacting protein 1; Atrophin-1-interacting protein A; KIAA0705activin receptor interacting protein 1; MAGI2; MAGI-2SSCAM; membrane associated guanylate kinase, WW and PDZ domain containing 2; Membrane-associated guanylate kinase inverted 2; membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2; SSCAM
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