Human/Mouse Neuronal Pentraxin R/NPTXR Antibody

Catalog #: MAB4414 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4414-100
MAB4414-SP

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Detection of Human and Mouse Neuronal Pentraxin R/NPTXR by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Neuronal Pentraxin R/NPTXR Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Neuronal Pentraxin R/NPTXR

Source

Monoclonal Mouse IgG_2B Clone # 511527

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human Neuronal Pentraxin R/NPTXR
Ala24-Ala500
Accession # O95502

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Human brain (hippocampus) and mouse brain (hippocampus)

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Neuronal Pentraxin R/NPTXR by Western Blot. Western blot shows lysates of human brain (hippocampus) and mouse brain (hippocampus). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Neuronal Pentraxin R/NPTXR Monoclonal Antibody (Catalog # MAB4414) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Neuronal Pentraxin R/NPTXR at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Neuronal Pentraxin R/NPTXR in Human Brain (Cortex). Neuronal Pentraxin R/NPTXR was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse Neuronal Pentraxin R/NPTXR Monoclonal Antibody (Catalog # MAB4414) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuronal Pentraxin R/NPTXR

The Neuronal Pentraxin Receptor (NPTXR; also called NPR) is a 55‑65 kDa, type II transmembrane glycoprotein within the Pentraxin family. NPTXR is co‑expressed and forms heteromultimers with the related, secreted proteins NPTX1 and NPTX2/NARP (1, 2). Human NPTXR is a 500 amino acid (aa) protein that includes a 477 aa extracellular domain (ECD) with one calcium-binding Pentraxin domain. The human NPTXR ECD shares 88% and 89% aa sequence identity with mouse and rat NPTXR, respectively. A 62 kDa soluble form is generated by TACE/ADAM17 proteolytic cleavage C-terminal to the transmembrane segment (3). Soluble forms of 55 kDa and 45 kDa have also been reported (2, 3). NPTXR is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions (1). It is enriched on axonal membranes at excitatory synapses where, with NPTX1, it recruits the GluR4 subunit of AMPA-type glutamate receptors (AMPAR) to promote synaptogenesis (3, 4). Synaptic activity stimulates mGluR1 or mGluR5 receptors, which then activate TACE, causing release of the NPTXR heteromultimer complex from the membrane (3). NPTX1/2 within the complex mediate binding AMPAR, while the soluble (but not transmembrane) form of NPTXR within the complex mediates AMPAR endocytosis and mGluR1/5‑mediated long-term depression (3). NPTXR is reported to be increased in cerebrospinal fluid or serum of Alzheimer’s disease patients as compared to controls (5). Aberrant expression of transmembrane NPTXR has also been reported in small-cell lung cancers (SCLC; 6).

References