Human/Mouse NGFRAP1/BEX3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse NGFRAP1/BEX3 by Western Blot.
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Product Details
Citations (1)
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Human/Mouse NGFRAP1/BEX3 Antibody Summary

Species Reactivity
Human, Mouse
Detects human NGFRAP1/BEX3 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 672911
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NGFRAP1/BEX3
Accession # Q00994
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse NGFRAP1/BEX3 antibody by Western Blot. View Larger

Detection of Human and Mouse NGFRAP1/BEX3 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGFRAP1/BEX3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry NGFRAP1/BEX3 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

NGFRAP1/BEX3 in Human Prostate. NGFRAP1/BEX3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGFRAP1/BEX3

NGFRAP1 (nerve growth factor receptor associated protein 1), also called BEX3 (brain-expressed X-linked protein 3), NADE (p75NTR-associated death executor) or FAP-1 (Fas-associated phosphatase-1), is a 24 kDa member of the BEX protein family. A nuclear export signal (aa 77-87) allows NGFRAP1 to export bound p75NTR. It is widely expressed, but highest in ovarian granulosa cells, testis, prostate, seminal vesicle and liver. Except for a 13-19 amino acid (aa) rodent Arg/His region at aa 35 that is not found in humans, human NGFRAP1 shares 94% aa identity with mouse and rat NGFRAP1. In humans, a 101 aa isoform with an alternative start site at aa 11 has been described.

Long Name
Nerve Growth Factor Receptor Associated Protein 1
Entrez Gene IDs
27018 (Human); 12070 (Mouse); 117089 (Rat)
Alternate Names
BEX3; BEX3p75NTR-associated cell death executor; Brain-expressed X-linked protein 3; DXS6984EX-linked 3; HGR74; NADE; nerve growth factor receptor (TNFRSF16) associated protein 1; Nerve growth factor receptor-associated protein 1; NGFRAP1; Ovarian granulosa cell 13.0 kDa protein HGR74; ovarian granulosa cell protein (13kD); protein BEX3

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Citation for Human/Mouse NGFRAP1/BEX3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. BEX3 contributes to cisplatin chemoresistance in nasopharyngeal carcinoma
    Authors: W Gao, JZ Li, SQ Chen, CY Chu, JY Chan, TS Wong
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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