Human/Mouse Pax3 /Pax7 Antibody
Human/Mouse Pax3 /Pax7 Antibody Summary
Met1-Ser215
Accession # NP_000429
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Pax3 in B16‑F1 Mouse Cell Line. Pax3 was detected in immersion fixed B16-F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax3/Pax7
Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.
Product Datasheets
Citations for Human/Mouse Pax3 /Pax7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Temporal analysis of enhancers during mouse cerebellar development reveals dynamic and novel regulatory functions
Authors: M Ramirez, Y Badayeva, J Yeung, J Wu, A Abdalla-Wy, E Yang, FANTOM 5 C, B Trost, SW Scherer, D Goldowitz
Elife, 2022;11(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
Cell Stem Cell, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
Cell Stem Cell, 2020;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC -
Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele
Authors: V Sahakyan, E Pozzo, R Duelen, J Deprest, M Sampaolesi
Stem Cells Int, 2017;2017(0):6101609.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
microRNA-155, induced by interleukin-1ss, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells.
Authors: Arts N, Cane S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, De Plaen E
PLoS ONE, 2015;10(4):e0122517.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers.
Authors: Plouhinec J, Roche D, Pegoraro C, Figueiredo A, Maczkowiak F, Brunet L, Milet C, Vert J, Pollet N, Harland R, Monsoro-Burq A
Dev Biol, 2014;386(2):461-72.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Cell-cycle dependent expression of a translocation-mediated fusion oncogene mediates checkpoint adaptation in rhabdomyosarcoma.
Authors: Kikuchi K, Hettmer S, Aslam M, Michalek J, Laub W, Wilky B, Loeb D, Rubin B, Wagers A, Keller C
PLoS Genet, 2014;10(1):e1004107.
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Lineage of origin in rhabdomyosarcoma informs pharmacological response.
Authors: Abraham J, Nunez-Alvarez Y, Hettmer S, Carrio E, Chen H, Nishijo K, Huang E, Prajapati S, Walker R, Davis S, Rebeles J, Wiebush H, McCleish A, Hampton S, Bjornson C, Brack A, Wagers A, Rando T, Capecchi M, Marini F, Ehler B, Zarzabal L, Goros M, Michalek J, Meltzer P, Langenau D, LeGallo R, Mansoor A, Chen Y, Suelves M, Rubin B, Keller C
Genes Dev, 2014;28(14):1578-91.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Functional dissection of Pax3 in paraxial mesoderm development and myogenesis.
Authors: Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R
Stem Cells, 2013;31(1):59-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
Authors: Awaya, Tomonari, Kato, Takeo, Mizuno, Yuta, Chang, Hsi, Niwa, Akira, Umeda, Katsutsu, Nakahata, Tatsutos, Heike, Toshio
PLoS ONE, 2012;7(12):e51638.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.
Authors: Gianakopoulos PJ, Mehta V, Voronova A, Cao Y, Yao Z, Coutu J, Wang X, Waddington MS, Tapscott SJ, Skerjanc IS
J. Biol. Chem., 2011;286(4):2517-25.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage.
Authors: Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC
Exp. Cell Res., 2008;314(8):1721-33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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