Human/Mouse Phospho-APP/Protease Nexin II (T668) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
APP/Protease Nexin II in Human Alzheimer's Disease Brain. APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 8 µg/mL Human/Mouse Phospho-APP/Protease Nexin II (T668) Monoclonal Antibody (Catalog # MAB2508) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Phospho-APP/Protease Nexin II (T668) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole for 16 hours. PVDF membrane was probed with 1 µg/mL of Human/Mouse Phospho-APP/Protease Nexin II (T668) Monoclonal Antibody (Catalog # MAB2508), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP-770, -751, and -733 contain a Kunitz protease inhibitor (KPI) domain (residue 291 - 342) and APP-695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta - and gamma -secretase cleave at the beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta ‑amyloid peptide (A beta 40 and A beta 42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha -secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).
- Haass, C. (2004) EMBO J. 23:483.
- Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
- Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
- Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human/Mouse Phospho-APP/Protease Nexin II (T668) Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Phospho-APP/Protease Nexin II (T668) Antibody and earn rewards!
Have you used Human/Mouse Phospho-APP/Protease Nexin II (T668) Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image